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- W2394171629 abstract "Objective To construct the plasmid of heavy chain of mice ferritin,and to research its possibility and superiority of being a new endogenous MRI reporter-ferritin. Methods The fragment of heavy chain of ferritin(Fth) was amplified from murine muscle mRNA using RT-PCR with a set of primers containing digestive sequences of restriction enzyme NotⅠ and BamHⅠ. The Fth fragment was inserted into pcDNA3.1 vector after digested with Not Ⅰ and BamH Ⅰ,and ligated with T4 ligase. Results The constructed plasmid was checked by digestion with restrictive enzymes and DNA sequencing. Conclusion The plasmid of heavy chain of murine can be successfully constructed." @default.
- W2394171629 created "2016-06-24" @default.
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- W2394171629 date "2009-01-01" @default.
- W2394171629 modified "2023-09-25" @default.
- W2394171629 title "Construction of a new endogenous MRI reporter gene of mice——the plasmid of ferritin H-chain gene" @default.
- W2394171629 hasPublicationYear "2009" @default.
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