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- W2394174350 abstract "AIM: To investigate the regulatory function of bone marrow-derived mesenchymal stem cells( MSCs) on T helper 17 cells( Th17) and regulatory T cells( Treg) in peripheral blood of severe asthmatic children.METHODS: MSCs were isolated,cultured and identified in vitro. MSCs digested with mitomycin were cocultured with T lymphocytes( TLC) at different ratios( 1∶ 1,1∶ 2,1∶ 10 and 1∶ 20) from severe asthmatic children for 72 h. The proliferation of TLC was measured by CCK-8 method. In the coculture system of the 1∶ 2 ratio and the single TLC system,the supernatant levels of interleukin-17( IL-17) and transforming growth factor-β( TGF-β) were measured by ELISA. The mRNA expression of retinoic acid-related orphan nuclear receptor C( RORC) and forkhead box protein 3( Foxp3) in TLC was detected by qRT-PCR. RESULTS: After cocultured with MSCs,the proliferation of TLC decreased significantly in a dosedependent manner( P 0. 05). It also showed decreases in IL-17( 3 799 ± 441 vs 4 890 ± 373,P 0. 05) and RORC mRNA level( 1. 21 ± 0. 14 vs 3. 85 ± 0. 48,P 0. 05),while an increase in TGF-β level( 209 ± 32 vs 117 ± 26,P 0. 05) was observed. No influence on the mRNA expression of Foxp3 was found( P 0. 05). CONCLUSION: MSCs suppresses Th17 polarization of naive peripheral blood CD4+T cells and matures Th17 cells secreting IL-17,which may effectively revise Th17 /Treg imbalance of asthma." @default.
- W2394174350 created "2016-06-24" @default.
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- W2394174350 date "2014-01-01" @default.
- W2394174350 modified "2023-09-23" @default.
- W2394174350 title "Bone marrow-derived mesenchymal stem cells regulate the function of Th17 / Treg in peripheral blood of severe asthmatic children" @default.
- W2394174350 hasPublicationYear "2014" @default.
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