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- W2394179771 abstract "In this study,the bait plasmid(pGBK-p24) with the full length of p24 encoded by Grapevine leafroll-associated virus 2 Liaoning isolate(GLRaV-2-LN),and grapevine cDNA library were constructed,and then grapevine cDNA library was screened by the bait plasmid to identify protein(s) that interact with p24.The results showed that the aa sequence and open reading frame of the insert cDNA fragment of p24 were correct,and the bait plasmid has no self-activating effect on yeast strain AH109 and Y187;The cDNA library had a titer of 2.2×106 cfu/ mL,and most insert fragments were more than 700 bp in size,which indicated that the cDNA library are qualified for the yeast two-hybrid screening.19 candidate postive clones displayed specific interaction with p24 and 16 cDNA sequences of these clones were obtained.BLAST showed that these cDNAs encode 8 proteins,such as MYC transcription factor,aconitase and type I inositol polyphosphate 5-phosphatase,etc;other three cDNA sequences encoded the same unnamed protein." @default.
- W2394179771 created "2016-06-24" @default.
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- W2394179771 date "2011-01-01" @default.
- W2394179771 modified "2023-09-23" @default.
- W2394179771 title "Preliminary study on interactions between p24 of Grapevine leafroll-associated virus 2 and the host by yeast two-hybrid screening" @default.
- W2394179771 hasPublicationYear "2011" @default.
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