Matches in SemOpenAlex for { <https://semopenalex.org/work/W2394188309> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2394188309 abstract "Objective: To clone p14 ARF gene from cells of the human normal liver cell line L02 and explore its inhibitory effect on tumor cell proliferation. Methods: p14 ARF cDNA was cloned from L02 cells by using RT-PCR method and inserted into the BamHI and EcoRI sites of pcDNA3 (called pcDNA3ARF). Lipofectamine mediated pcDNA3ARF was transferred into tumor cell lines, such as Hep2, KB, KB-v200, HepG2, HLE, Huh7, MCF7 and MCF7/ADR. The number of colonies of these tumor cell lines were calculated after more than two weeks' screening in DMEM medium containing G418. Results:The colony-forming efficiency of the p14ARF -transfected KB-v200 and MCF7/ADR drug-resistant cells was 13±2.0 and 21±1.5 , respectively, compared with 86±3.1(15%)and 126±7.5(17%)in the cells transfected with empty vector pcDNA3, and in the p53 positive tumor cell lines, such as HepG2(47%), Hep2(57%), MCF7(58%) cells, the inhibition was also found,whereas in the p53 negative tumor cell lines, such as KB(74%), HLE(65%), Huh-7(80%) cells, the inhibitory effect was milder. Conclusions: p14 ARF can suppress the growth of the p53 positive tumor cell lines in a p53-relevant manner, while in the p53 negative, drug-resistant tumor cells, p14 ARF has strong inhibitory effect, indicating the inhibitory effect of p14 ARF on growth of drug-resistant tumor cells is independent on p53, at least in KB-v200 and MCF7/ADR drug-resistant cells." @default.
- W2394188309 created "2016-06-24" @default.
- W2394188309 creator A5007128842 @default.
- W2394188309 date "2000-01-01" @default.
- W2394188309 modified "2023-09-25" @default.
- W2394188309 title "Transfer of p14~(ARF) gene in human tumor cell lines inhibits cell proliferation" @default.
- W2394188309 hasPublicationYear "2000" @default.
- W2394188309 type Work @default.
- W2394188309 sameAs 2394188309 @default.
- W2394188309 citedByCount "0" @default.
- W2394188309 crossrefType "journal-article" @default.
- W2394188309 hasAuthorship W2394188309A5007128842 @default.
- W2394188309 hasConcept C104317684 @default.
- W2394188309 hasConcept C1491633281 @default.
- W2394188309 hasConcept C153911025 @default.
- W2394188309 hasConcept C185592680 @default.
- W2394188309 hasConcept C2776750431 @default.
- W2394188309 hasConcept C2778264664 @default.
- W2394188309 hasConcept C2779707156 @default.
- W2394188309 hasConcept C40767141 @default.
- W2394188309 hasConcept C49923114 @default.
- W2394188309 hasConcept C502942594 @default.
- W2394188309 hasConcept C54009773 @default.
- W2394188309 hasConcept C54355233 @default.
- W2394188309 hasConcept C55493867 @default.
- W2394188309 hasConcept C555283112 @default.
- W2394188309 hasConcept C62112901 @default.
- W2394188309 hasConcept C81089528 @default.
- W2394188309 hasConcept C81885089 @default.
- W2394188309 hasConcept C86803240 @default.
- W2394188309 hasConcept C92087593 @default.
- W2394188309 hasConceptScore W2394188309C104317684 @default.
- W2394188309 hasConceptScore W2394188309C1491633281 @default.
- W2394188309 hasConceptScore W2394188309C153911025 @default.
- W2394188309 hasConceptScore W2394188309C185592680 @default.
- W2394188309 hasConceptScore W2394188309C2776750431 @default.
- W2394188309 hasConceptScore W2394188309C2778264664 @default.
- W2394188309 hasConceptScore W2394188309C2779707156 @default.
- W2394188309 hasConceptScore W2394188309C40767141 @default.
- W2394188309 hasConceptScore W2394188309C49923114 @default.
- W2394188309 hasConceptScore W2394188309C502942594 @default.
- W2394188309 hasConceptScore W2394188309C54009773 @default.
- W2394188309 hasConceptScore W2394188309C54355233 @default.
- W2394188309 hasConceptScore W2394188309C55493867 @default.
- W2394188309 hasConceptScore W2394188309C555283112 @default.
- W2394188309 hasConceptScore W2394188309C62112901 @default.
- W2394188309 hasConceptScore W2394188309C81089528 @default.
- W2394188309 hasConceptScore W2394188309C81885089 @default.
- W2394188309 hasConceptScore W2394188309C86803240 @default.
- W2394188309 hasConceptScore W2394188309C92087593 @default.
- W2394188309 hasLocation W23941883091 @default.
- W2394188309 hasOpenAccess W2394188309 @default.
- W2394188309 hasPrimaryLocation W23941883091 @default.
- W2394188309 hasRelatedWork W2336934942 @default.
- W2394188309 hasRelatedWork W2349945840 @default.
- W2394188309 hasRelatedWork W2365141962 @default.
- W2394188309 hasRelatedWork W2369496453 @default.
- W2394188309 hasRelatedWork W2373228689 @default.
- W2394188309 hasRelatedWork W2396143836 @default.
- W2394188309 hasRelatedWork W2396384146 @default.
- W2394188309 hasRelatedWork W2399298769 @default.
- W2394188309 hasRelatedWork W2412146115 @default.
- W2394188309 hasRelatedWork W2415675129 @default.
- W2394188309 hasRelatedWork W2469347963 @default.
- W2394188309 hasRelatedWork W2497346098 @default.
- W2394188309 hasRelatedWork W2809710754 @default.
- W2394188309 hasRelatedWork W2920826237 @default.
- W2394188309 hasRelatedWork W3009016903 @default.
- W2394188309 hasRelatedWork W3029880360 @default.
- W2394188309 hasRelatedWork W3030238769 @default.
- W2394188309 hasRelatedWork W3030377454 @default.
- W2394188309 hasRelatedWork W3031289199 @default.
- W2394188309 hasRelatedWork W3031541580 @default.
- W2394188309 isParatext "false" @default.
- W2394188309 isRetracted "false" @default.
- W2394188309 magId "2394188309" @default.
- W2394188309 workType "article" @default.