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- W2394201724 abstract "PurposeTo optimize the culture condition of CPU-04301 bacterial, and to find out a method to assay the enzyme activity of D-aminoacylase.MethodsThe carbon source and nitrogen source of culture of CPU-04301 bacterial were optimized. Then the effects of temperature, pH, and concentration of metal ions on the test of enzyme activity were studied. ResultsUsing immobilized CPU-04301, product 4.78 g were obtained by enzymatic reaction at 37 ℃ for 10 h. The purity of product was 99.57%.ConclusionAfter being optimized, the yield and enzyme activity of CPU-04301 increased markedly. An accuracy and sensitive assay method of D-aminoacylase was established." @default.
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- W2394201724 date "2004-01-01" @default.
- W2394201724 modified "2023-09-24" @default.
- W2394201724 title "Study on producing D-amino acid in series by immobilized cell" @default.
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