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- W2394276553 abstract "To make a definite diagnosis for a special case of suspected rabies patient,a series of laboratory examinations including fluorescent antibody assay(FA),ELISA and RT-PCR were carried out to detect the presence of rabies virus in the brain tissues of the suspected rabies patient,as well as in the brain tissues of suckling mice that were inoculated with the brain suspension of the suspected rabies patient.It was demonstrated that the negative results in direct FA assay and the double antibody sandwich ELISA were found,but the result in RT-PCR was positive.In addition,the brain tissues of suckling mice inoculated with brain tissue of the suspected rabies patient showed positive results in these 3 methods of detection mentioned above.As demonstrated by the nucleoprotein gene sequence of the RT-PCR product of the isolated virus and comparison of the phylogenetic relation with Mega 3.1 software,the isolated virus was found to be the gene type 1 rabies virus and this virus showed close relation to the viruses isolated in the same region previously.From these observations,it is concluded that this suspected case can be definitely diagnosed as rabies,and the major and decisive cause for the death of this patient is rabies,but the complicated pulmonary infection would hasten his death.As claimed by WHO,the FA assay is the golden standard for the diagnosis of rabies,but acording to the present study,it is clear that definite diagnosis can only be achieved by the combined use of various laboratory investigations,in which both the virus inoculation and RT-PCR might be more sensitive than SA assay." @default.
- W2394276553 created "2016-06-24" @default.
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- W2394276553 date "2008-01-01" @default.
- W2394276553 modified "2023-09-23" @default.
- W2394276553 title "Laboratory examination and confirmation for a special case of suspected rabies patient" @default.
- W2394276553 hasPublicationYear "2008" @default.
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