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- W2394277605 abstract "To investigate the expression of gene encoding the EM II/3 antigen of Echinococcus multilocularis in E.coli and BCG,the recombinant plasmid pBCG-EM II/3 was transformed into the susceptible strain of E.coli Top10,and introduced to BCG by electroporation to construct the recombinant BCG.The bacteria containing the recombinants were cultivated to the logarithm growth period and induced by heating at 45℃.Meanwhile,the expressed products were analyzed by SDS-PAGE and Western blotting respectively.The experimental results showed that the plasmid pBCG-Em II/3 could successfully express the EM II/3 protein in BCG.which was identified as a band of 65 kDa as demonstrated by SDS-PAGE analysis,and the content of EM II/3 protein accounted for 9% of the total bacterial protein of BCG,This protein could bind with specific antibodies in mouse sera.However,the same kind of protein was not expressed in E.coli.It is concluded that the recombinant protein EM II/3 can be expressed in BCG and this kind of protein shows specific antigenicity." @default.
- W2394277605 created "2016-06-24" @default.
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- W2394277605 date "2006-01-01" @default.
- W2394277605 modified "2023-09-28" @default.
- W2394277605 title "Expression of gene encoding the EMII/3 antigen of Echinococcus multilocularis in Escherichia coli and BCG" @default.
- W2394277605 hasPublicationYear "2006" @default.
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