Matches in SemOpenAlex for { <https://semopenalex.org/work/W2394328287> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2394328287 abstract "Gluteryl-7-aminocephalosporanic acid (GL-7-ACA) acylase (EC 3.1.5.11 ), one of the important industrial enzyme used in the manufacture of 7-aminocephalosporanic acid (7-ACA ) through the two-step enzymatic process, catalyses efficient bioconversion of GL-7-ACA to 7-ACA. The objective of this work was to study the production of GL-7-ACA acylase using genetic engineering strain, Escherichia coli MMR204 harboring recombinant plasmid pZC1 grown in batch and fed-batch culture on LB medium supplemented with glucose. It was found that the optimal temperature and pH in the fermentation for enzyme production were 33℃ and 7.5～8.5,respectively. The initial concentration of glucose ranging between 1 and 5 g/L facilitate the increase of both biomass and enzyme productivity. When the concentration of glucose added reached to 6g/L, culture broth in the end of fermentation was acidic (pH 4.0) without GL-7-ACA acylase formation. In the comparison of three kinds of feeding mode for glucose fed-batch operation in 5L fermentor, it was demonstrated that the acy gene was constitutively expressed under the control of the tac-promoter without induction and the exponential feeding gave the highest yield of both the biomass and enzyme activity, but the expression efficiency of acy gene with pH8.5-constant control mode in the fed-batch culture was slightly higher than exponential or steady rate mode." @default.
- W2394328287 created "2016-06-24" @default.
- W2394328287 creator A5013516483 @default.
- W2394328287 date "2004-01-01" @default.
- W2394328287 modified "2023-09-25" @default.
- W2394328287 title "Optimization of Glucose Fed-batch Culture for GL-7-ACA Acylase Production by Escherichia coli MMR204/pZC1" @default.
- W2394328287 hasPublicationYear "2004" @default.
- W2394328287 type Work @default.
- W2394328287 sameAs 2394328287 @default.
- W2394328287 citedByCount "0" @default.
- W2394328287 crossrefType "journal-article" @default.
- W2394328287 hasAuthorship W2394328287A5013516483 @default.
- W2394328287 hasConcept C100544194 @default.
- W2394328287 hasConcept C104317684 @default.
- W2394328287 hasConcept C105702510 @default.
- W2394328287 hasConcept C134121241 @default.
- W2394328287 hasConcept C167431671 @default.
- W2394328287 hasConcept C168170006 @default.
- W2394328287 hasConcept C178790620 @default.
- W2394328287 hasConcept C181199279 @default.
- W2394328287 hasConcept C185592680 @default.
- W2394328287 hasConcept C191897082 @default.
- W2394328287 hasConcept C192562407 @default.
- W2394328287 hasConcept C2778022156 @default.
- W2394328287 hasConcept C2780074830 @default.
- W2394328287 hasConcept C43617362 @default.
- W2394328287 hasConcept C547475151 @default.
- W2394328287 hasConcept C55493867 @default.
- W2394328287 hasConcept C67568554 @default.
- W2394328287 hasConcept C86803240 @default.
- W2394328287 hasConceptScore W2394328287C100544194 @default.
- W2394328287 hasConceptScore W2394328287C104317684 @default.
- W2394328287 hasConceptScore W2394328287C105702510 @default.
- W2394328287 hasConceptScore W2394328287C134121241 @default.
- W2394328287 hasConceptScore W2394328287C167431671 @default.
- W2394328287 hasConceptScore W2394328287C168170006 @default.
- W2394328287 hasConceptScore W2394328287C178790620 @default.
- W2394328287 hasConceptScore W2394328287C181199279 @default.
- W2394328287 hasConceptScore W2394328287C185592680 @default.
- W2394328287 hasConceptScore W2394328287C191897082 @default.
- W2394328287 hasConceptScore W2394328287C192562407 @default.
- W2394328287 hasConceptScore W2394328287C2778022156 @default.
- W2394328287 hasConceptScore W2394328287C2780074830 @default.
- W2394328287 hasConceptScore W2394328287C43617362 @default.
- W2394328287 hasConceptScore W2394328287C547475151 @default.
- W2394328287 hasConceptScore W2394328287C55493867 @default.
- W2394328287 hasConceptScore W2394328287C67568554 @default.
- W2394328287 hasConceptScore W2394328287C86803240 @default.
- W2394328287 hasLocation W23943282871 @default.
- W2394328287 hasOpenAccess W2394328287 @default.
- W2394328287 hasPrimaryLocation W23943282871 @default.
- W2394328287 hasRelatedWork W1971942406 @default.
- W2394328287 hasRelatedWork W1979606958 @default.
- W2394328287 hasRelatedWork W1997619834 @default.
- W2394328287 hasRelatedWork W2023881801 @default.
- W2394328287 hasRelatedWork W2064158183 @default.
- W2394328287 hasRelatedWork W2081960345 @default.
- W2394328287 hasRelatedWork W2095733110 @default.
- W2394328287 hasRelatedWork W2113482993 @default.
- W2394328287 hasRelatedWork W2214971100 @default.
- W2394328287 hasRelatedWork W2317026107 @default.
- W2394328287 hasRelatedWork W2415558038 @default.
- W2394328287 hasRelatedWork W2913172117 @default.
- W2394328287 hasRelatedWork W3088829452 @default.
- W2394328287 hasRelatedWork W3164481650 @default.
- W2394328287 hasRelatedWork W5485897 @default.
- W2394328287 hasRelatedWork W587576083 @default.
- W2394328287 hasRelatedWork W90714065 @default.
- W2394328287 hasRelatedWork W181284019 @default.
- W2394328287 hasRelatedWork W2185416887 @default.
- W2394328287 hasRelatedWork W2829844866 @default.
- W2394328287 isParatext "false" @default.
- W2394328287 isRetracted "false" @default.
- W2394328287 magId "2394328287" @default.
- W2394328287 workType "article" @default.