Matches in SemOpenAlex for { <https://semopenalex.org/work/W2394328489> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2394328489 abstract "Objective To establish the method for isolation and cultivation of mesenchymal stem cells(MSCs) from human embryo in vitro,and to identify their biological characteristics. Methods Cells were separated from human embryo 5-6 weeks after digestion with 0.25% trypsin for 7-10 min,and the anchoring cells were expanded in MSCs media.Flow cytometry(FCM) was adopted to determine the immunophenotypes of MSCs,and the proliferation ability and cell cycle were detected by MTT and propidium iodide(PI) staining. Different induction conditions were used to identify the multipotent differentiation.Results MSCs were obtained from human embryo by tissue digestion. FCM result showed the immunophenotype of MSCs was positive for CD29,CD44,CD73,CD105 and CD166,and negative for CD34,CD45 and HLA-DR.The doubling time of MSCs was 23 h.Lipid droplets were found in lipo-induced MSCs,and under ossa-induced condition,the histological analysis of the cells revealed stronger expression of alkatnephosphatase and the mineralization of calcium salts was confirmed by alizarin red . Conclusion The phenotype of MSCs derived from human early embryo is coincident with adult MSCs,and these cells have the potential to differentiate into adipocytes and osteoblasts." @default.
- W2394328489 created "2016-06-24" @default.
- W2394328489 creator A5002948434 @default.
- W2394328489 date "2007-01-01" @default.
- W2394328489 modified "2023-09-23" @default.
- W2394328489 title "Isolation and cultivation of mesenchymal stem cells derived from human embryo in vitro and their biological characteristics" @default.
- W2394328489 hasPublicationYear "2007" @default.
- W2394328489 type Work @default.
- W2394328489 sameAs 2394328489 @default.
- W2394328489 citedByCount "0" @default.
- W2394328489 crossrefType "journal-article" @default.
- W2394328489 hasAuthorship W2394328489A5002948434 @default.
- W2394328489 hasConcept C10205521 @default.
- W2394328489 hasConcept C116716535 @default.
- W2394328489 hasConcept C153911025 @default.
- W2394328489 hasConcept C16685009 @default.
- W2394328489 hasConcept C185592680 @default.
- W2394328489 hasConcept C190283241 @default.
- W2394328489 hasConcept C196166836 @default.
- W2394328489 hasConcept C198826908 @default.
- W2394328489 hasConcept C202751555 @default.
- W2394328489 hasConcept C2775934118 @default.
- W2394328489 hasConcept C2780932548 @default.
- W2394328489 hasConcept C28328180 @default.
- W2394328489 hasConcept C31573885 @default.
- W2394328489 hasConcept C553184892 @default.
- W2394328489 hasConcept C55493867 @default.
- W2394328489 hasConcept C71924100 @default.
- W2394328489 hasConcept C86803240 @default.
- W2394328489 hasConcept C95444343 @default.
- W2394328489 hasConceptScore W2394328489C10205521 @default.
- W2394328489 hasConceptScore W2394328489C116716535 @default.
- W2394328489 hasConceptScore W2394328489C153911025 @default.
- W2394328489 hasConceptScore W2394328489C16685009 @default.
- W2394328489 hasConceptScore W2394328489C185592680 @default.
- W2394328489 hasConceptScore W2394328489C190283241 @default.
- W2394328489 hasConceptScore W2394328489C196166836 @default.
- W2394328489 hasConceptScore W2394328489C198826908 @default.
- W2394328489 hasConceptScore W2394328489C202751555 @default.
- W2394328489 hasConceptScore W2394328489C2775934118 @default.
- W2394328489 hasConceptScore W2394328489C2780932548 @default.
- W2394328489 hasConceptScore W2394328489C28328180 @default.
- W2394328489 hasConceptScore W2394328489C31573885 @default.
- W2394328489 hasConceptScore W2394328489C553184892 @default.
- W2394328489 hasConceptScore W2394328489C55493867 @default.
- W2394328489 hasConceptScore W2394328489C71924100 @default.
- W2394328489 hasConceptScore W2394328489C86803240 @default.
- W2394328489 hasConceptScore W2394328489C95444343 @default.
- W2394328489 hasLocation W23943284891 @default.
- W2394328489 hasOpenAccess W2394328489 @default.
- W2394328489 hasPrimaryLocation W23943284891 @default.
- W2394328489 hasRelatedWork W186366613 @default.
- W2394328489 hasRelatedWork W2045363008 @default.
- W2394328489 hasRelatedWork W2060599105 @default.
- W2394328489 hasRelatedWork W2063314748 @default.
- W2394328489 hasRelatedWork W2349825191 @default.
- W2394328489 hasRelatedWork W2362333151 @default.
- W2394328489 hasRelatedWork W2363126019 @default.
- W2394328489 hasRelatedWork W2363991590 @default.
- W2394328489 hasRelatedWork W2364687812 @default.
- W2394328489 hasRelatedWork W2366456360 @default.
- W2394328489 hasRelatedWork W2367517929 @default.
- W2394328489 hasRelatedWork W2373666379 @default.
- W2394328489 hasRelatedWork W2374771411 @default.
- W2394328489 hasRelatedWork W2377678485 @default.
- W2394328489 hasRelatedWork W2379747647 @default.
- W2394328489 hasRelatedWork W2379976280 @default.
- W2394328489 hasRelatedWork W2381636833 @default.
- W2394328489 hasRelatedWork W2389388695 @default.
- W2394328489 hasRelatedWork W2409336843 @default.
- W2394328489 hasRelatedWork W3151693881 @default.
- W2394328489 isParatext "false" @default.
- W2394328489 isRetracted "false" @default.
- W2394328489 magId "2394328489" @default.
- W2394328489 workType "article" @default.