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- W2394333668 abstract "The hPPAR-LBD coding sequence was amplified with RT-PCR from the total RNA of human breast cancer cell line MCF-7,comfirmed by nucleotide sequencing, and then cloned into prokaryotic expression vector pGEX-1γT. The recombinant expression vector was transformed into E.coli strain BL21(DE3), and the expression of fusion protein GST-hPPAR-LBD was induced by IPTG. Under different culture temperatures or induced IPTG concentrations, the fusion protein was soluble or insoluble (inclusion bodies), and was purified by glutathione-Sephorose 4B affinity chromatography. The purified fusion protein bound its natural ligand specifically, and was also recognized by specific anti-hPPAR polyantibodies." @default.
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- W2394333668 date "2001-01-01" @default.
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- W2394333668 title "THE EXPRESSION,PURIFUCATION AND DETECTION OF PPARγ-LIGAND BINDING DOMAIN" @default.
- W2394333668 hasPublicationYear "2001" @default.
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