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- W2394337792 abstract "Purpose:To explore the morphological changes of type A spermatogonia whose telomerase activity were repressed by antisense oligodeoxynucleotide(ASODN).Methods:Phosphorothioate mTR antisence ODNs was encapsulated by liposomes LipofectAMINE 2000(LF 2000) and transfected to Sprague Dawley (SD) rat spermatosonia. The inhibition of telomerase activity was detected aided by Bioluminescence technique. The morphological changes of type A spermatogonia was studied with microscope and electron microscope.Results:mTR ASODN conjugated with LipofectAMINE could significantly inhibit telomerase activity of spermatgonia(P 0.05). Significant morphological changes could be observed in some spermatgonia with the aid of microscope and electron microscope. No morphological change of telomerase activity were observed in the control term either Liposome or SODN.Conclusions:mTR ASODN could significantly inhibit telomerase activity of spermatgonia. Significant morphological changes were observed in some spermatgonia when telomerase activity was repressed." @default.
- W2394337792 created "2016-06-24" @default.
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- W2394337792 date "2003-01-01" @default.
- W2394337792 modified "2023-09-23" @default.
- W2394337792 title "Purification of type A spermatogonia in vitro and its morphological changes after inhibition of telomerase activity" @default.
- W2394337792 hasPublicationYear "2003" @default.
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