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- W2394351630 abstract "Objectives To isolate and identify Brucella and provide scientific evidence for diagnosis,treatment and con trol of Brucellosis.Methods Rose-Bengal plate agglutination test(RBPT)、standard tube agglutination test(SAT)、enzyme linked immunosorbent assay(ELISA) were used to test specific antibody in the sera.Serum agglutination test、Bacteriophage test and polymerase chain reaction(PCR) were used to identify the type of stains.Results Flocculent antigen-antibody combo 100% agglutination appeared in RBPT was positive;The result of SAT was positive(1:200,++ +);The serum IgM antibody was positive and IgG was negative during the course of disease;Genomes were extracted from M5 Brucella vaccine strains and the patient strain.After amplification and electrophoresis,all appeared positive banding around 223 and 731 bp fragments and no banding at 711,976 and 285 bp.It indicated that the strain isolated from the patient was B.melitensis;After stained with CO2,H2S,bacteriostasis,strain surface antigen agglutination and phage cracking test,the strain was identified as B.melitensis type Ⅲ.Conclusion The pathogen of first case was B.melitensis type Ⅲ in Suizhou.The monitoring work of brucellosis be strengthened.RBPT,SAT、ELISA and PCR allowed rapidly detection of Brucella and they can provide evidence for clinical di agnosis and control the prevalence of brucellosis." @default.
- W2394351630 created "2016-06-24" @default.
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- W2394351630 date "2013-01-01" @default.
- W2394351630 modified "2023-09-27" @default.
- W2394351630 title "Pathogenic detection of the first cases of B.melitensis type III in Suizhou city" @default.
- W2394351630 hasPublicationYear "2013" @default.
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