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- W2394361946 abstract "Objective:To investigate the effect of Hirsutella sinensis(HS) on the expression of transforming growth factor-β1(TGF-β1) and fibronectin(FN) in rat peritoneal mesothelial cells(RPMCs) cultured in high glucose concentration in vitro.Methods:The third generation RPMCs from primary culture were divided randomly into 6 groups:control group,1.5% glucose group,2.5% glucose group,HS(10 mg/ml) group,1.5% glucose+HS(10 mg/ml) group,2.5% glucose+HS(10 mg/ml) group.After 72 h,the mRNA and protein expression of TGF-β1 and FN were determined by RT-PCR and ELISA respectively.Results:The mRNA and protein expression of TGF-β1 and FN increased significantly in RPMCs cultured with high glucose,compared with control group(P0.05),and accompanying with the increased concentration of glucose.As compared with high glucose group(1.5% or 2.5% glucose),the mRNA and protein expression of TGF-β1 and FN decreased significantly in high glucose with Hirsutella sinensis (10 mg/ml) group(P0.05).Conclusion:Hirsutella sinensis reduces the expression of TGF-β1 and FN induced by high glucose in RPMCs,and has the inhibited effect on peritoneal fibrosis." @default.
- W2394361946 created "2016-06-24" @default.
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- W2394361946 date "2011-01-01" @default.
- W2394361946 modified "2023-09-23" @default.
- W2394361946 title "Effects of Hirsutella Sinensis on the Expression of Transforming Growth Factor-β_1 and Fibronectin in Rat Peritoneal Mesothelial Cells Cultured in High Glucose Concentration" @default.
- W2394361946 hasPublicationYear "2011" @default.
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