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- W2394365893 abstract "Objective To investigate the effect of a new cationic carrier polyethylenimine(PEI) on transfection of the aquaporin 5(AQP5) gene into A549 cells in vitro.Methods According to different N/P ratios(5,10,15,20 and 25),the eukaryotic expression vector pEGFP-N1 was mixed with the green fluorescent protein(GFP) and PEI to make a transfection complex,using lipofectamine2000 as the positive control.Transfection efficiency was detected by flow cytometry for optimizing the optimal N/P ratio.Full-length AQP5 cDNA were cloned into the pEGFP-N1 vector to construct pEGFP-N-AQP5,which was then transfected into A549 cells.Expression of AQP5 in transfected cells was detected using PCR and Western blot.Results Flow cytometry revealed the GFP emitting.When the N/P ratio was 15,the PEI/pEGFP-N1 complex achieved the highest transfection efficiency of 89.72%,close to 91.44% when delivered by liposomes.Compared with the control group,the AQP5 gene and protein expressions were significantly increased after successful transfection of pEGFP-N-AQP5 into A549 by application of the PEI and lipofectamine2000 complex.Conclusion pEGFP-N-AQP5 can be successfully transfected into A549 cells mediated by the novel cationic carrier PEI." @default.
- W2394365893 created "2016-06-24" @default.
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- W2394365893 date "2011-01-01" @default.
- W2394365893 modified "2023-09-27" @default.
- W2394365893 title "Transfection of the AQP5 gene mediated by a novel cational carrier PEI in vitro" @default.
- W2394365893 hasPublicationYear "2011" @default.
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