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- W2394367905 abstract "Objective To clone and construct the pGEM-T-S vector containing the translational initiation region of poly(ADP-ribose)polymerase(hPARP-1) gene cDNA with two recongnition sites for the enzymes BamH Ⅰ and Sac Ⅰ,then compare its sequence with Genbank and provide experimental material for subclone and toxicological study.Methods Following to total RNA being extracted from human embryo lung fibroblast and RT-PCR being carried out,T vector was linked with PCR products.After E.coil DH5α was transformed with recombitants and screened with blue/white blot test for positive clones from which plasmide were then extracted,the recombinants were identified by PCR, restriction analysis and sequencing.Results 507 base pairs fragment contraining restriction sites was obtained through RT-PCR.After colning by T vector and being identified by restriction analysis and sequening,it was confirmed that the object fragment had 99^9% homology with hPARP-1 sequence in Genbank.Conclusion The T vector clone with the translational initiation region of hPARP-1 gene cDNA has been constructed successfully.This clone can be used in research subclone and PARP-1 gene deficiency cell." @default.
- W2394367905 created "2016-06-24" @default.
- W2394367905 creator A5038894201 @default.
- W2394367905 date "2003-01-01" @default.
- W2394367905 modified "2023-09-24" @default.
- W2394367905 title "The translational initiation region of hPARP-1 gene cDNA cloning by T vector" @default.
- W2394367905 hasPublicationYear "2003" @default.
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