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- W2394406581 abstract "Purpose To express soluble and functional human APO2L in Escherichia coli and refold it into a functional form. Methods Human APO2L condons 114-281 were amplified by polymerase chain reaction and subcloned into expression vector.The product was transfected into E.coli JF1125 and expressed by temperature inducement in shaking bottle.The selected high expression clone was expressed in bioreactor.Soluble human APO2L was obtained by a protocol,which includes dissolving by Gu·HCl,refolding by Air Oxidation,diafiltration and Q?Sepharose ino exchange chromatography. Results SDS?PAGE analysis revealed that expression product was Mr.2.1,about 20% of total bacteria protein and formed inclusion body.The purity of soluble human APO2L was more than 90%.Induction of Apoptosis by the product became conscious in Hela Cells. Conclusions The product expressed in Escherichia coli has similar activity to that generated in cells." @default.
- W2394406581 created "2016-06-24" @default.
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- W2394406581 date "2000-01-01" @default.
- W2394406581 modified "2023-09-25" @default.
- W2394406581 title "Molecular Cloning and Expression of Human Soluble APO2L/TRAIL Gene in Escherichia coli" @default.
- W2394406581 hasPublicationYear "2000" @default.
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