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- W2394442714 abstract "The 3-month-old Jian common carps(Cyprinus carpio var.Jian) were exposed to various concentrations of Cd~(2+)(0.005,0.05,0.5,5 mg/L) for two days,and no individuals died within the two-day exposure.Also,no significantly amplified loci were found in both treatment and control fishes when ten RAPD primers were detected for the difference in genome DNA.However,when methylation sensitive amplified polymorphism(MSAP) was applied to analyze methylation changes in genome DNA of muscle,significant changes of methylation in CCGG sites occurred in the fish after 2 days of 5 mg/L Cd~(2+) exposure.Furthermore,most fish in this group died within one month.In the groups exposed to 0.05 mg/L and 0.5 mg/L for two days,a slight decline in the proportion of methylation loci were observed,but no obvious change in methylation pattern in comparison with the controls.This study shows that the methyltion of Jian carp in CCGG sites of genome DNA was relatively stable during exposure to various concentrations of Cd~(2+).At low Cd~(2+) concentration the Cd~(2+) physiological toxicity might be reduced by Jian common carp with fine-tuning in methylated sites,while at high Cd~(2+) concentration,significant changes would occur in methylation sites of genome DNA,possibly leading to an abnormal expression of some silent genes.Fig 2,Tab 2,Ref 31" @default.
- W2394442714 created "2016-06-24" @default.
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- W2394442714 date "2010-01-01" @default.
- W2394442714 modified "2023-09-25" @default.
- W2394442714 title "Effect of Cadmium at Different Concentrations on Genomic DNA of Common Carps" @default.
- W2394442714 hasPublicationYear "2010" @default.
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