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- W2394471552 abstract "Horizontal starch gel electrophoresis was used to investigate the tissue-specific differentiation and genetic variation between two wild stocks of the tongue sole (.Cynoglossus semilaevis. Guntuer) collected from the Yellow Sea and the Bohai Sea. A preliminary screening for 18 isozymes in nine different tissues and organs (gills, skeleton muscle, liver, eye, heart, spleen, kidney, brain and dorsal fin) was carried out using TC-7.0 buffer system. The results showed that the isozyme expression remarkably presented tissue-specific in .C.semilaevis.. Based on the preliminary screening, thirteen isozymes (PGDH, MPI, IDHP, SOD, AAT, MDH, LDH, G3PDH, PGM, LAP, ADH, SDH and ALP) in two tissues (skeleton muscle and liver) were employed to analyze the genetic structure of .C.semilaevis .stocks. In total, 16 putative enzyme-coding loci were detected. As detected, four loci (IDHP*、PGM*、G3PDH*、SDH*) presented polymorphic in the Yellow Sea stock, while five loci (IDHP*、PGM*、MDH-2*、SDH*、ALP*) were detected in the Bohai Sea stock. The proportions of polymorphic loci of the Yellow Sea and the Bohai Sea stock were 0.250 0 and 0.312 5, respectively. The mean values of the observed heterozygosities in these two stocks were 0.014 3 and 0.010 4 with the expected heterozygosities of 0.014 0 and 0.010 2, respectively, while the average effective numbers of alleles were 1.015 1 and 1.010 8, respectively. The Nei’s genetic distance between these two stocks was 0.000 12, which suggested that genetic diversity in this species be relatively low without significant genetic divergence between the geographic stocks." @default.
- W2394471552 created "2016-06-24" @default.
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- W2394471552 date "2006-01-01" @default.
- W2394471552 modified "2023-09-22" @default.
- W2394471552 title "Genetic diversities in the tongue sole (Cynoglossus semilaevis. Günther) as revealed by isozyme analysis" @default.
- W2394471552 hasPublicationYear "2006" @default.
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