Matches in SemOpenAlex for { <https://semopenalex.org/work/W2394552218> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2394552218 endingPage "H612" @default.
- W2394552218 startingPage "H606" @default.
- W2394552218 abstract "Compounds that elevate intraplatelet guanosine 3',5'-cyclic monophosphate (cGMP) or adenosine 3',5'-cyclic monophosphate (cAMP) stimulate the phosphorylation of a 46- to 56-kDa thrombocyte protein designated “vasodilator-stimulated protein” (VASP), which is most likely involved in the regulation of adhesion/aggregation. We investigated whether endothelium-derived relaxing factor (EDRF)/nitric oxide (NO) and prostaglandin I2 (PGI2) affected VASP phosphorylation in washed human platelets that were injected into the coronaries of saline-perfused rabbit hearts (n = 22) and collected immediately after passage. The endothelial stimulator acetylcholine (ACh; 1 microM) significantly increased the concentration of cGMP (indicating release of EDRF) and PGI2 in the coronary venous effluent, as well as the concentration of cGMP and cAMP in platelets. Phosphorylation state of VASP increased from 32.1 +/- 2.9 to 64.8 +/- 2.7%. Inhibition of EDRF/NO synthesis by NG-nitro-L-arginine (30 microM) completely abolished the ACh-induced cGMP increase, attenuated the cAMP-elevation without affecting PGI2, and caused a 20.5 +/- 5.8% decrease of the phosphorylation state of VASP. Indomethacin (30 microM) virtually abolished ACh-induced increases of PGI2, cAMP (but not cGMP), and phosphorylated VASP. These results indicate that both EDRF/NO and PGI2 contribute to the regulation of VASP phosphorylation in platelets collected after a single coronary passage. Their synergistic inhibitory effects on platelet function may thus be mediated by a common effect on target proteins like VASP as well as by a secondary increase in cAMP in response to cGMP-elevating compounds such as EDRF." @default.
- W2394552218 created "2016-06-24" @default.
- W2394552218 creator A5045033843 @default.
- W2394552218 creator A5047275885 @default.
- W2394552218 creator A5069089776 @default.
- W2394552218 creator A5070560020 @default.
- W2394552218 creator A5072719814 @default.
- W2394552218 date "1994-02-01" @default.
- W2394552218 modified "2023-09-27" @default.
- W2394552218 title "Endothelium-dependent phosphorylation of vasodilator-stimulated protein in platelets during coronary passage" @default.
- W2394552218 doi "https://doi.org/10.1152/ajpheart.1994.266.2.h606" @default.
- W2394552218 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8141362" @default.
- W2394552218 hasPublicationYear "1994" @default.
- W2394552218 type Work @default.
- W2394552218 sameAs 2394552218 @default.
- W2394552218 citedByCount "6" @default.
- W2394552218 countsByYear W23945522182013 @default.
- W2394552218 crossrefType "journal-article" @default.
- W2394552218 hasAuthorship W2394552218A5045033843 @default.
- W2394552218 hasAuthorship W2394552218A5047275885 @default.
- W2394552218 hasAuthorship W2394552218A5069089776 @default.
- W2394552218 hasAuthorship W2394552218A5070560020 @default.
- W2394552218 hasAuthorship W2394552218A5072719814 @default.
- W2394552218 hasConcept C11960822 @default.
- W2394552218 hasConcept C120770815 @default.
- W2394552218 hasConcept C126322002 @default.
- W2394552218 hasConcept C134018914 @default.
- W2394552218 hasConcept C170493617 @default.
- W2394552218 hasConcept C185592680 @default.
- W2394552218 hasConcept C2776668983 @default.
- W2394552218 hasConcept C2776991684 @default.
- W2394552218 hasConcept C2776992346 @default.
- W2394552218 hasConcept C2777995097 @default.
- W2394552218 hasConcept C2780994212 @default.
- W2394552218 hasConcept C2781128415 @default.
- W2394552218 hasConcept C2909076160 @default.
- W2394552218 hasConcept C519581460 @default.
- W2394552218 hasConcept C55493867 @default.
- W2394552218 hasConcept C71924100 @default.
- W2394552218 hasConcept C86803240 @default.
- W2394552218 hasConcept C87325107 @default.
- W2394552218 hasConcept C89560881 @default.
- W2394552218 hasConcept C97029542 @default.
- W2394552218 hasConceptScore W2394552218C11960822 @default.
- W2394552218 hasConceptScore W2394552218C120770815 @default.
- W2394552218 hasConceptScore W2394552218C126322002 @default.
- W2394552218 hasConceptScore W2394552218C134018914 @default.
- W2394552218 hasConceptScore W2394552218C170493617 @default.
- W2394552218 hasConceptScore W2394552218C185592680 @default.
- W2394552218 hasConceptScore W2394552218C2776668983 @default.
- W2394552218 hasConceptScore W2394552218C2776991684 @default.
- W2394552218 hasConceptScore W2394552218C2776992346 @default.
- W2394552218 hasConceptScore W2394552218C2777995097 @default.
- W2394552218 hasConceptScore W2394552218C2780994212 @default.
- W2394552218 hasConceptScore W2394552218C2781128415 @default.
- W2394552218 hasConceptScore W2394552218C2909076160 @default.
- W2394552218 hasConceptScore W2394552218C519581460 @default.
- W2394552218 hasConceptScore W2394552218C55493867 @default.
- W2394552218 hasConceptScore W2394552218C71924100 @default.
- W2394552218 hasConceptScore W2394552218C86803240 @default.
- W2394552218 hasConceptScore W2394552218C87325107 @default.
- W2394552218 hasConceptScore W2394552218C89560881 @default.
- W2394552218 hasConceptScore W2394552218C97029542 @default.
- W2394552218 hasIssue "2" @default.
- W2394552218 hasLocation W23945522181 @default.
- W2394552218 hasLocation W23945522182 @default.
- W2394552218 hasOpenAccess W2394552218 @default.
- W2394552218 hasPrimaryLocation W23945522181 @default.
- W2394552218 hasRelatedWork W2017104343 @default.
- W2394552218 hasRelatedWork W2022160223 @default.
- W2394552218 hasRelatedWork W2040231821 @default.
- W2394552218 hasRelatedWork W2048292033 @default.
- W2394552218 hasRelatedWork W2064708176 @default.
- W2394552218 hasRelatedWork W2067815977 @default.
- W2394552218 hasRelatedWork W2162526763 @default.
- W2394552218 hasRelatedWork W2394552218 @default.
- W2394552218 hasRelatedWork W2412948052 @default.
- W2394552218 hasRelatedWork W3023584569 @default.
- W2394552218 hasVolume "266" @default.
- W2394552218 isParatext "false" @default.
- W2394552218 isRetracted "false" @default.
- W2394552218 magId "2394552218" @default.
- W2394552218 workType "article" @default.