FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2394604804> ?p ?o ?g. }

• W2394604804 endingPage "4" @default.
• W2394604804 startingPage "690" @default.
• W2394604804 abstract "To study the chiral selectivity in vascular endothelial differentiation in drug resistant lung cancer cells induced by high-dose L- or D-methotrexate (MTX) enantiomer.Human lung cancer cells of the line A549 were co-cultured with high-dose (15 micromol/L) L- or D-MTX enantiomer so as to develop cancer cells resistant to MTX. MTT method was used to detect the drug resistant index. Flow cytometry was used to detect the expression of CD44, a transmembrane glycoprotein reflecting the migration ability of cells, CD31, a marker of vascular endothelium, and P-170 protein. Fifteen BALB/c nude mice were inoculated with the parent A549 cells, L-MTX-resistant A549 cells induced by L-MTX enantiomer, and D-MTX-resistant A549 cells induced by D-MTX enantiomer. Four weeks later the mice were killed to take out the tumor masses. Immunohistochemistry with CD34 staining was used to detect the microvascular density (MVD).The drug resistant index of the D-MTX induced drug resistant A549 cells was 20.1 +/- 2.3, significantly higher than that of the L-MTX-induced cells (12.4 +/- 1.2, P = 0.000). The CD44 positive rate of the D-MTX induced A549 cells was 97.0% +/- 0.9%, not significantly different from that of the L-MTX-induced A549 cells (96.7% +/- 1.4%, P = 0.544). The CD31 positive rate of the D-MTX induced A549 cells was 91.9% +/- 3.2%, significantly higher than that of the L-MTX-induced A549 cells (32.9% +/- 8.0%, P = 0.000). The P-170 protein positive rate of the parent cells was 85.5% +/- 4.6%, and the P-170 protein positive rate of the D-MTX-induced A549 cells was 87.0% +/- 8.9%, significantly higher than that of the L-MTX-induced cells (71.5% +/- 8.2%, P = 0.002). The MVD of the D-MTX-induced cells was 55.9 +/- 11.9, significantly higher than that of the L-MTX-induced cells (7.2 +/- 1.7, P = 0.000). MVD was significantly positively correlated with the CD31 level (r = 0.462, P = 0.007), and not correlated with P-170 protein and CD34 levels.The MTX enantiomers have different chiral selectivity on human lung cancer cell, D-MTX resistant cells shows a potential of differentiation from cancer cells to vascular endothelial cells. D-MTX is not be regarded just as a pollutant in the drug MTX, MTX with single enantiomer (L-MTX) should be selected clinically so as to decrease the side effects of D-MTX." @default.
• W2394604804 created "2016-06-24" @default.
• W2394604804 creator A5019853987 @default.
• W2394604804 creator A5021384155 @default.
• W2394604804 creator A5045440240 @default.
• W2394604804 creator A5073123246 @default.
• W2394604804 creator A5076130185 @default.
• W2394604804 creator A5078079654 @default.
• W2394604804 creator A5087473873 @default.
• W2394604804 date "2009-03-17" @default.
• W2394604804 modified "2023-10-17" @default.
• W2394604804 title "[Chiral selectivity in differentiation of lung cancer A549 cell to vascular endothelial cells after drug resistance induced by D-or L-methotrexate enantiomers]." @default.
• W2394604804 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19595065" @default.
• W2394604804 hasPublicationYear "2009" @default.
• W2394604804 type Work @default.
• W2394604804 sameAs 2394604804 @default.
• W2394604804 citedByCount "0" @default.
• W2394604804 crossrefType "journal-article" @default.
• W2394604804 hasAuthorship W2394604804A5019853987 @default.
• W2394604804 hasAuthorship W2394604804A5021384155 @default.
• W2394604804 hasAuthorship W2394604804A5045440240 @default.
• W2394604804 hasAuthorship W2394604804A5073123246 @default.
• W2394604804 hasAuthorship W2394604804A5076130185 @default.
• W2394604804 hasAuthorship W2394604804A5078079654 @default.
• W2394604804 hasAuthorship W2394604804A5087473873 @default.
• W2394604804 hasConcept C126322002 @default.
• W2394604804 hasConcept C1491633281 @default.
• W2394604804 hasConcept C153911025 @default.
• W2394604804 hasConcept C185592680 @default.
• W2394604804 hasConcept C202751555 @default.
• W2394604804 hasConcept C203014093 @default.
• W2394604804 hasConcept C2776256026 @default.
• W2394604804 hasConcept C2778939058 @default.
• W2394604804 hasConcept C2780932548 @default.
• W2394604804 hasConcept C2781059491 @default.
• W2394604804 hasConcept C502942594 @default.
• W2394604804 hasConcept C55493867 @default.
• W2394604804 hasConcept C71924100 @default.
• W2394604804 hasConcept C81729549 @default.
• W2394604804 hasConcept C86803240 @default.
• W2394604804 hasConcept C98274493 @default.
• W2394604804 hasConceptScore W2394604804C126322002 @default.
• W2394604804 hasConceptScore W2394604804C1491633281 @default.
• W2394604804 hasConceptScore W2394604804C153911025 @default.
• W2394604804 hasConceptScore W2394604804C185592680 @default.
• W2394604804 hasConceptScore W2394604804C202751555 @default.
• W2394604804 hasConceptScore W2394604804C203014093 @default.
• W2394604804 hasConceptScore W2394604804C2776256026 @default.
• W2394604804 hasConceptScore W2394604804C2778939058 @default.
• W2394604804 hasConceptScore W2394604804C2780932548 @default.
• W2394604804 hasConceptScore W2394604804C2781059491 @default.
• W2394604804 hasConceptScore W2394604804C502942594 @default.
• W2394604804 hasConceptScore W2394604804C55493867 @default.
• W2394604804 hasConceptScore W2394604804C71924100 @default.
• W2394604804 hasConceptScore W2394604804C81729549 @default.
• W2394604804 hasConceptScore W2394604804C86803240 @default.
• W2394604804 hasConceptScore W2394604804C98274493 @default.
• W2394604804 hasIssue "10" @default.
• W2394604804 hasLocation W23946048041 @default.
• W2394604804 hasOpenAccess W2394604804 @default.
• W2394604804 hasPrimaryLocation W23946048041 @default.
• W2394604804 hasRelatedWork W1254814353 @default.
• W2394604804 hasRelatedWork W2053210732 @default.
• W2394604804 hasRelatedWork W2079954978 @default.
• W2394604804 hasRelatedWork W2104749832 @default.
• W2394604804 hasRelatedWork W2117786645 @default.
• W2394604804 hasRelatedWork W2158373964 @default.
• W2394604804 hasRelatedWork W2159800348 @default.
• W2394604804 hasRelatedWork W2352089118 @default.
• W2394604804 hasRelatedWork W2352644336 @default.
• W2394604804 hasRelatedWork W2365093469 @default.
• W2394604804 hasRelatedWork W2371952436 @default.
• W2394604804 hasRelatedWork W2373838495 @default.
• W2394604804 hasRelatedWork W2374685724 @default.
• W2394604804 hasRelatedWork W2401102293 @default.
• W2394604804 hasRelatedWork W2413339122 @default.
• W2394604804 hasRelatedWork W2438378456 @default.
• W2394604804 hasRelatedWork W2744099366 @default.
• W2394604804 hasRelatedWork W2994020154 @default.
• W2394604804 hasRelatedWork W3028634060 @default.
• W2394604804 hasRelatedWork W3032030251 @default.
• W2394604804 hasVolume "89" @default.
• W2394604804 isParatext "false" @default.
• W2394604804 isRetracted "false" @default.
• W2394604804 magId "2394604804" @default.
• W2394604804 workType "article" @default.