Matches in SemOpenAlex for { <https://semopenalex.org/work/W2394672225> ?p ?o ?g. }
- W2394672225 endingPage "1189" @default.
- W2394672225 startingPage "1179" @default.
- W2394672225 abstract "Abstract Oxygen plays a key role in stem cell biology as a signaling molecule and as an indicator of cell energy metabolism. Quantification of cellular oxygen kinetics, i.e. the determination of specific oxygen uptake rates (sOURs), is routinely used to understand metabolic shifts. However current methods to determine sOUR in adherent cell cultures rely on cell sampling, which impacts on cellular phenotype. We present real‐time monitoring of cell growth from phase contrast microscopy images, and of respiration using optical sensors for dissolved oxygen. Time‐course data for bulk and peri‐cellular oxygen concentrations obtained for Chinese hamster ovary (CHO) and mouse embryonic stem cell (mESCs) cultures successfully demonstrated this non‐invasive and label‐free approach. Additionally, we confirmed non‐invasive detection of cellular responses to rapidly changing culture conditions by exposing the cells to mitochondrial inhibiting and uncoupling agents. For the CHO and mESCs, sOUR values between 8 and 60 amol cell −1 s −1 , and 5 and 35 amol cell −1 s −1 were obtained, respectively. These values compare favorably with literature data. The capability to monitor oxygen tensions, cell growth, and sOUR, of adherent stem cell cultures, non‐invasively and in real time, will be of significant benefit for future studies in stem cell biology and stem cell‐based therapies." @default.
- W2394672225 created "2016-06-24" @default.
- W2394672225 creator A5003795307 @default.
- W2394672225 creator A5042278068 @default.
- W2394672225 creator A5042308700 @default.
- W2394672225 creator A5055860157 @default.
- W2394672225 creator A5056135069 @default.
- W2394672225 creator A5076935643 @default.
- W2394672225 creator A5083987006 @default.
- W2394672225 date "2016-06-22" @default.
- W2394672225 modified "2023-10-16" @default.
- W2394672225 title "Real-time monitoring of specific oxygen uptake rates of embryonic stem cells in a microfluidic cell culture device" @default.
- W2394672225 cites W1561765789 @default.
- W2394672225 cites W1965653791 @default.
- W2394672225 cites W1969162184 @default.
- W2394672225 cites W1971063609 @default.
- W2394672225 cites W1974515589 @default.
- W2394672225 cites W1975211161 @default.
- W2394672225 cites W1975371675 @default.
- W2394672225 cites W1978018545 @default.
- W2394672225 cites W1980236358 @default.
- W2394672225 cites W1981343389 @default.
- W2394672225 cites W1985197198 @default.
- W2394672225 cites W1987783568 @default.
- W2394672225 cites W1989240379 @default.
- W2394672225 cites W1991372299 @default.
- W2394672225 cites W1993805976 @default.
- W2394672225 cites W1997920550 @default.
- W2394672225 cites W2001961551 @default.
- W2394672225 cites W2003430967 @default.
- W2394672225 cites W2010408511 @default.
- W2394672225 cites W2011892782 @default.
- W2394672225 cites W2016915024 @default.
- W2394672225 cites W2022894624 @default.
- W2394672225 cites W2024907483 @default.
- W2394672225 cites W2025192477 @default.
- W2394672225 cites W2028264366 @default.
- W2394672225 cites W2028876849 @default.
- W2394672225 cites W2031328542 @default.
- W2394672225 cites W2032707537 @default.
- W2394672225 cites W2034595317 @default.
- W2394672225 cites W2040374671 @default.
- W2394672225 cites W2043253996 @default.
- W2394672225 cites W2043668209 @default.
- W2394672225 cites W2050198980 @default.
- W2394672225 cites W2050719916 @default.
- W2394672225 cites W2051531582 @default.
- W2394672225 cites W2053153356 @default.
- W2394672225 cites W2055608980 @default.
- W2394672225 cites W2057131748 @default.
- W2394672225 cites W2058580259 @default.
- W2394672225 cites W2059783442 @default.
- W2394672225 cites W2061941238 @default.
- W2394672225 cites W2065174416 @default.
- W2394672225 cites W2066004242 @default.
- W2394672225 cites W2066174543 @default.
- W2394672225 cites W2068835257 @default.
- W2394672225 cites W2070160283 @default.
- W2394672225 cites W2070563987 @default.
- W2394672225 cites W2072198755 @default.
- W2394672225 cites W2073481357 @default.
- W2394672225 cites W2075429958 @default.
- W2394672225 cites W2076994662 @default.
- W2394672225 cites W2079855250 @default.
- W2394672225 cites W2084037323 @default.
- W2394672225 cites W2091780534 @default.
- W2394672225 cites W2092564795 @default.
- W2394672225 cites W2096433224 @default.
- W2394672225 cites W2096769057 @default.
- W2394672225 cites W2104953790 @default.
- W2394672225 cites W2107596763 @default.
- W2394672225 cites W2114662808 @default.
- W2394672225 cites W2117336213 @default.
- W2394672225 cites W2118456668 @default.
- W2394672225 cites W2119126373 @default.
- W2394672225 cites W2125169325 @default.
- W2394672225 cites W2125436483 @default.
- W2394672225 cites W2133486230 @default.
- W2394672225 cites W2136047046 @default.
- W2394672225 cites W2138803549 @default.
- W2394672225 cites W2143594562 @default.
- W2394672225 cites W2143751064 @default.
- W2394672225 cites W2150419930 @default.
- W2394672225 cites W2158246101 @default.
- W2394672225 cites W2162428762 @default.
- W2394672225 cites W2164417692 @default.
- W2394672225 cites W2165240683 @default.
- W2394672225 cites W2169413134 @default.
- W2394672225 cites W2170861130 @default.
- W2394672225 cites W2171803108 @default.
- W2394672225 doi "https://doi.org/10.1002/biot.201500479" @default.
- W2394672225 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5103178" @default.
- W2394672225 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27214658" @default.
- W2394672225 hasPublicationYear "2016" @default.
- W2394672225 type Work @default.
- W2394672225 sameAs 2394672225 @default.
- W2394672225 citedByCount "36" @default.
- W2394672225 countsByYear W23946722252017 @default.