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- W2394873072 abstract "The oil containing high oleic acid is high nutritional. In Brassica napus, the fatty acid desaturase gene (FAD2) is the key gene controlling oleic acid content. In this study, the full-length cDNA sequences of three genes located on chromosome A5, C5 and A1 in Brassica napus were cloned and named BnFAD2-A5, BnFAD2-C5 and BnFAD2-A1. The three genes encode proteins with 384, 384, and 136 amino acid residues, respectively. TMHMM was used to predict transmembrane domain and Clust X software was used to analyze the activity center of FAD2 genes. Both of the results showed that BnFAD2-A1 did not have the function of dehydrogenase. The yeast complementary experiment on four genes (including published BnFAD2-C1 gene) showed that the desaturation capability of BnFAD2-A5 gene was next to that of BnFAD2-C5 gene, and both of them were greater than that of BnFAD2-C1 gene. The expression patterns of the four genes were analyzed by using qRT-PCR technique in different tissues and the protein stability of BnFAD2-C1, BnFAD2-A5, and BnFAD2-C5 was analyzed by using Hemagglutinin labeling method. Both of the results revealed that BnFAD2-A5 and BnFAD2-C5 are major genes affecting the accumulation of oleic acid in B. napus." @default.
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- W2394873072 date "2016-01-01" @default.
- W2394873072 modified "2023-10-18" @default.
- W2394873072 title "Cloning and Expression Analyses for BnFAD2 Genes in Brassica napus" @default.
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- W2394873072 doi "https://doi.org/10.3724/sp.j.1006.2016.01000" @default.
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