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• W2394966935 abstract "AbstractThe principle of commonly used methods to create mutations in the nematode Caenorhabditis elegans (C. elegans) is straightforward. In general, worms are exposed to a dose of mutagen resulting in DNA damages and mutations. Screening the progeny of the mutagenized animals for a certain phenotype is the regular forward genetic approach in C. elegans. A mutant selected from such a population is stabilized to recover a pure homozygous strain. In this chapter, we categorize the protocol into mutagenesis, phenotype screen, and outcross and provide time-tested procedures for their implementation to create long-lived worm mutants.Key words Caenorhabditis elegans MutagenesisLifespanScreenForward geneticsMutationOutcross" @default.
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• W2394966935 date "2013-01-01" @default.
• W2394966935 modified "2023-10-18" @default.
• W2394966935 title "Methods for Creating Mutations in C. elegans That Extend Lifespan" @default.
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• W2394966935 doi "https://doi.org/10.1007/978-1-62703-556-9_6" @default.
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