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- W2395160987 abstract "High-resolution 31P nuclear magnetic reso- nance spectra at 145.7 MHz are reported for intact Ehrlich as- cites tumor cells and their perchloric acid extracts. In the ex- tracts it was possible to assign resonances to fructose 1,6-bis- phosphates, dihydroxyacetone phosphate, ATP, ADP, AMP, Pi, NAD+, phosphorylcholine, glycero-3-phosphorylcholine, glyc- ero-3-phosphorylethanolamine, and glyceraldehyde 3-phosphate from their chemical shifts, pH behavior, and spin couplings. All but glyceraldehyde 3-phosphate were observed and assigned in the intact cells. It was possible to show that the hydrolysis of fructose 1,6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate is in equilibrium, that the dihy- droxyacetone phosphate -. glyceraldehyde 3-phosphate reaction is not, and that in the intact cell without added oxygen or glucose the reaction 2ADP >z? ATP + AMP is in equilibrium. From the known pH dependence of the Pi resonance it was possible to show that during aerobic or anerobic glycolysis the difference between intracellular and extracellular pH values was <0.2 pH units. Upon oxygenation the ATP concentration increased while the ADP concentration fell. Introducing deoxyglucose depleted the ATP and resulted in an AMP signal and one from deoxy- glucose 6-phosphate, which is transported and phosphorylated but not catabolized. _~~~~~ B (c)" @default.
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- W2395160987 date "2016-01-01" @default.
- W2395160987 modified "2023-09-26" @default.
- W2395160987 title "31P Nuclear magnetic resonance studies of Ehrlich ascites" @default.
- W2395160987 hasPublicationYear "2016" @default.
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