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• W2395170935 abstract "Background. A large number of screening tests for rapid diagnosis of Urinary Tract Infection UTI is used in the Clinical Laboratory but each of them is questionable and an ideal screening test for diagnosis of UTI is not available at present. Aim of this study is to evaluate the feasibility of a screening for rapid diagnosis of UTI by using a second generation flow cytometer. The authors evaluated the analytical performance of the Sysmex UF-100 cytometer versus the UTI diagnosis made on the basis of laboratory and clinical data. Materials and Methods. We considered 2010 consecutive subjects, aged between 18 and 78, 870 males and 1140 females, whose recently collected urine samples were submitted to our Laboratory for microbiological examination. The majority (90.2% ) of the samples were voided urine specimens collected by using the midstream technique. The samples were collected in sterile containers and a 12 mL aliquot was transferred into test tubes and analyzed within one hour. Each sample was submitted to microbiological evaluation (culture + RAA), dipstick tests, UF-100 examination and microscopic observation. These results were considered together with the clinical data and patients characteristics to obtain a final diagnosis of UTI. The analytical performance of the Laboratory tests was obtained by using this diagnosis as a standard. Results. At first the authors evaluated the analytical performance of the quantitative detection of pyuria by using the UF-100 cytometer to assess the cut-off value. After determination of this cut-off value at 25 White Blood Cells (WBC)/mL, the authors compared the performance of the UF-100 with the dipstick screening and the microscopic examination of unstained centrifuged urine by using a cut-off at 10 WBC/HPF. Of the considered 2010 subjects 529 (26.32%) were clinically diagnosed as UTI. The UF-100 based screening gave as results SE=0.90, SP=0.94, PPV=0.83, NPV=0.96, and CCI=0.93. Conclusions. In our experience the results of the UF-100 based screening in the diagnosis of UTI in adult patients are better than those obtained from classical microscopic examination of unstained centrifuged urine and/or those of the dipstick screening test. Moreover, the quantification of pyuria, used in this study as a screening test for UTI, is not a new test to be introduced in the Clinical Laboratory practice with additional cost, but it is rather a by-product of the examination of the corpuscled portion of urine by a flow cytometer." @default.
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• W2395170935 date "2001-01-01" @default.
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• W2395170935 title "Measurement of urine leukocytes by a second generation flow cytometer; application in the diagnosis of acute urinary tract infections in adult patients" @default.
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