Matches in SemOpenAlex for { <https://semopenalex.org/work/W2395234231> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2395234231 endingPage "16" @default.
- W2395234231 startingPage "309" @default.
- W2395234231 abstract "The cDNA of IgE constant domain of rat was cloned from the spleen of allergy asthma rat by RT-PCR. The IL-1ra segment was obtained from intermediate vector pBV220-IL-1ra. By overlap extension PCR, the fusion gene IL-1ra-Fcepsilon was cloned, then inserted into the eukaryotic expression plasmid pIRES2-EGFP to obtain a recombinant expression plasmid pIRES2-EGFP-IL-1ra-Fcepsilon. The recombinant expression plasmid was transfected into 293T cells using lipofectamin and instillated into the rat lung through trachea. The expression of IL-1ra-Fcepsilon was identified by Western blot, RT-PCR, and this protein could inhibit the activity of IL-1 in vitro. Green fluorescent protein could be detected in the transfected 293T cells and the rat lungs at different times. The research paved the way for the gene therapy of allergy asthma." @default.
- W2395234231 created "2016-06-24" @default.
- W2395234231 creator A5011251160 @default.
- W2395234231 creator A5029818269 @default.
- W2395234231 creator A5049131158 @default.
- W2395234231 creator A5074832223 @default.
- W2395234231 creator A5083519576 @default.
- W2395234231 date "2008-08-01" @default.
- W2395234231 modified "2023-09-23" @default.
- W2395234231 title "[Construction and identification of the eukaryotic expression vector pIRES2-EGFP-IL-1ra-Fcepsilon]." @default.
- W2395234231 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18959005" @default.
- W2395234231 hasPublicationYear "2008" @default.
- W2395234231 type Work @default.
- W2395234231 sameAs 2395234231 @default.
- W2395234231 citedByCount "1" @default.
- W2395234231 countsByYear W23952342312018 @default.
- W2395234231 crossrefType "journal-article" @default.
- W2395234231 hasAuthorship W2395234231A5011251160 @default.
- W2395234231 hasAuthorship W2395234231A5029818269 @default.
- W2395234231 hasAuthorship W2395234231A5049131158 @default.
- W2395234231 hasAuthorship W2395234231A5074832223 @default.
- W2395234231 hasAuthorship W2395234231A5083519576 @default.
- W2395234231 hasConcept C104317684 @default.
- W2395234231 hasConcept C111829193 @default.
- W2395234231 hasConcept C123894998 @default.
- W2395234231 hasConcept C142613039 @default.
- W2395234231 hasConcept C150194340 @default.
- W2395234231 hasConcept C153911025 @default.
- W2395234231 hasConcept C174510640 @default.
- W2395234231 hasConcept C187882448 @default.
- W2395234231 hasConcept C22744801 @default.
- W2395234231 hasConcept C2776415932 @default.
- W2395234231 hasConcept C33161422 @default.
- W2395234231 hasConcept C40767141 @default.
- W2395234231 hasConcept C54009773 @default.
- W2395234231 hasConcept C54355233 @default.
- W2395234231 hasConcept C86803240 @default.
- W2395234231 hasConcept C92087593 @default.
- W2395234231 hasConceptScore W2395234231C104317684 @default.
- W2395234231 hasConceptScore W2395234231C111829193 @default.
- W2395234231 hasConceptScore W2395234231C123894998 @default.
- W2395234231 hasConceptScore W2395234231C142613039 @default.
- W2395234231 hasConceptScore W2395234231C150194340 @default.
- W2395234231 hasConceptScore W2395234231C153911025 @default.
- W2395234231 hasConceptScore W2395234231C174510640 @default.
- W2395234231 hasConceptScore W2395234231C187882448 @default.
- W2395234231 hasConceptScore W2395234231C22744801 @default.
- W2395234231 hasConceptScore W2395234231C2776415932 @default.
- W2395234231 hasConceptScore W2395234231C33161422 @default.
- W2395234231 hasConceptScore W2395234231C40767141 @default.
- W2395234231 hasConceptScore W2395234231C54009773 @default.
- W2395234231 hasConceptScore W2395234231C54355233 @default.
- W2395234231 hasConceptScore W2395234231C86803240 @default.
- W2395234231 hasConceptScore W2395234231C92087593 @default.
- W2395234231 hasIssue "4" @default.
- W2395234231 hasLocation W23952342311 @default.
- W2395234231 hasOpenAccess W2395234231 @default.
- W2395234231 hasPrimaryLocation W23952342311 @default.
- W2395234231 hasRelatedWork W2350640915 @default.
- W2395234231 hasRelatedWork W2350999683 @default.
- W2395234231 hasRelatedWork W2353838075 @default.
- W2395234231 hasRelatedWork W2356830185 @default.
- W2395234231 hasRelatedWork W2358943378 @default.
- W2395234231 hasRelatedWork W2360867898 @default.
- W2395234231 hasRelatedWork W2362332041 @default.
- W2395234231 hasRelatedWork W2364827421 @default.
- W2395234231 hasRelatedWork W2369231681 @default.
- W2395234231 hasRelatedWork W2371118012 @default.
- W2395234231 hasRelatedWork W2371788052 @default.
- W2395234231 hasRelatedWork W2374451795 @default.
- W2395234231 hasRelatedWork W2379629732 @default.
- W2395234231 hasRelatedWork W2382726535 @default.
- W2395234231 hasRelatedWork W2384323851 @default.
- W2395234231 hasRelatedWork W2385565520 @default.
- W2395234231 hasRelatedWork W2387805344 @default.
- W2395234231 hasRelatedWork W2388565905 @default.
- W2395234231 hasRelatedWork W3029599149 @default.
- W2395234231 hasRelatedWork W3031084822 @default.
- W2395234231 hasVolume "41" @default.
- W2395234231 isParatext "false" @default.
- W2395234231 isRetracted "false" @default.
- W2395234231 magId "2395234231" @default.
- W2395234231 workType "article" @default.