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- W2395238049 abstract "ABSTRACT Rabbit polyclonal antibodies were raised against β-glucuronidase purified from mouse liver. This antiserum immunoprecipitated the β-glucuronidase secreted by mouse fibroblasts but did not cross-react with the same enzyme isolated from human tissue. The β-glucuronidase present in mouse 3T3 fibroblasts and mouse peritoneal macrophages was clearly identified by indirect immunofluorescence, using the antiserum and an FITC-conjugated second antibody, while human fibroblasts with normal levels of β-glucuronidase activity did not fluoresce when tested with the same reagents. A range of human fibroblasts, human neuroblastoma and rat glioma cells did not fluoresce when incubated with the antibody but did fluoresce after they had been co-cultured for 24 h with mouse macrophages, showing that mouse β-glucuronidase had been transferred from adherent macrophages into adjacent recipient cells. Transfer took place even when receptor-mediated endocytosis was blocked with a suitable competitive ligand, the transferred enzyme being visible mainly as a bright punctate fluorescence with a lysosome-like distribution. Macrophages thus have the potential to act as donors of lysosomal enzymes to a wide range of recipient cells and to transfer enzymes to them during direct cell-to-cell contact." @default.
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- W2395238049 date "1985-11-01" @default.
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- W2395238049 title "Direct transfer of β-glucuronidase from mouse macrophages to other types of cell" @default.
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- W2395238049 doi "https://doi.org/10.1242/jcs.79.1.137" @default.
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