Matches in SemOpenAlex for { <https://semopenalex.org/work/W2395297185> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2395297185 endingPage "7" @default.
- W2395297185 startingPage "581" @default.
- W2395297185 abstract "Antibodies directed against guanosin-8-yl-acetylaminofluorene have been employed in a sensitive radioimmunoassay able to distinguish between the acetylated and deacetylated deoxyguanosine C-8 adducts of 2-acetylaminofluorene. Most cultured cells (primary BALB/c and Sencar epidermal and fibroblast cells and primary rat epidermal cells and fibroblasts) form ≥90% of the total C-8 adducts as the deacetylated adduct after a 1-h exposure to 10-5 M N-acetoxy-2-acetylaminofluorene. However, primary rat hepatocytes, exposed to either the activated derivative of the carcinogen for 1 h or the parent compound 2-acetylaminofluorene for 5-24 h, form ≥80% of the C-8 adducts as the acetylated adduct. Treatment conditions were varied in order to alter the levels of binding and proportion of acetylated and deacetylated C-8 adducts formed in primary BALB/c epidermal cells. Cells exposed in medium 199 with 1.2 mM Ca2+ in the presence of 10% fetal calf serum (standard culture conditions) bind 100-200 fmol/µg DNA and form 3% of the C-8 adducts as acetylated. Exposure to carcinogen in the absence of serum increased binding levels two- to fivefold with 8% of the C-8 adducts in the acetylated form. The addition of 10-5 M ethidium bromide (a DNA intercalating agent), 10-3 M sodium butyrate (a deacetylase inhibitor), or 10-5 M harman (which augments 2-acetylaminofluorene mutagenesis) failed to change the level or pattern of binding. Carcinogen exposure under culture conditions which select for the growing fraction of epidermal cells (0.09 mM Ca2+) also failed to alter binding. However, a 20-min pretreatment with 10-5 M paraoxon under standard culture conditions resulted in the inhibition of 99% of the binding and formation of deacetylated adduct. The small amount of C-8 adduct formed was acetylated. These results indicate that the extent and pattern of 2-acetylaminofluorene DNA binding vary in different cell types and can be altered by variations in exposure conditions. By these means it should be possible to investigate the biological importance of individual adducts." @default.
- W2395297185 created "2016-06-24" @default.
- W2395297185 creator A5021425054 @default.
- W2395297185 creator A5025822048 @default.
- W2395297185 creator A5026839393 @default.
- W2395297185 date "1980-11-01" @default.
- W2395297185 modified "2023-10-12" @default.
- W2395297185 title "Effect of culture conditions, cell type, and species of origin on the distribution of acetylated and deacetylated deoxyguanosine C-8 adducts of N-acetoxy-2-acetylaminofluorene." @default.
- W2395297185 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7464821" @default.
- W2395297185 hasPublicationYear "1980" @default.
- W2395297185 type Work @default.
- W2395297185 sameAs 2395297185 @default.
- W2395297185 citedByCount "12" @default.
- W2395297185 crossrefType "journal-article" @default.
- W2395297185 hasAuthorship W2395297185A5021425054 @default.
- W2395297185 hasAuthorship W2395297185A5025822048 @default.
- W2395297185 hasAuthorship W2395297185A5026839393 @default.
- W2395297185 hasConcept C104317684 @default.
- W2395297185 hasConcept C108204754 @default.
- W2395297185 hasConcept C114246631 @default.
- W2395297185 hasConcept C119157956 @default.
- W2395297185 hasConcept C153911025 @default.
- W2395297185 hasConcept C178790620 @default.
- W2395297185 hasConcept C185592680 @default.
- W2395297185 hasConcept C202751555 @default.
- W2395297185 hasConcept C2779838395 @default.
- W2395297185 hasConcept C2780381497 @default.
- W2395297185 hasConcept C2780441211 @default.
- W2395297185 hasConcept C552990157 @default.
- W2395297185 hasConcept C55493867 @default.
- W2395297185 hasConcept C71240020 @default.
- W2395297185 hasConcept C86803240 @default.
- W2395297185 hasConcept C87644729 @default.
- W2395297185 hasConceptScore W2395297185C104317684 @default.
- W2395297185 hasConceptScore W2395297185C108204754 @default.
- W2395297185 hasConceptScore W2395297185C114246631 @default.
- W2395297185 hasConceptScore W2395297185C119157956 @default.
- W2395297185 hasConceptScore W2395297185C153911025 @default.
- W2395297185 hasConceptScore W2395297185C178790620 @default.
- W2395297185 hasConceptScore W2395297185C185592680 @default.
- W2395297185 hasConceptScore W2395297185C202751555 @default.
- W2395297185 hasConceptScore W2395297185C2779838395 @default.
- W2395297185 hasConceptScore W2395297185C2780381497 @default.
- W2395297185 hasConceptScore W2395297185C2780441211 @default.
- W2395297185 hasConceptScore W2395297185C552990157 @default.
- W2395297185 hasConceptScore W2395297185C55493867 @default.
- W2395297185 hasConceptScore W2395297185C71240020 @default.
- W2395297185 hasConceptScore W2395297185C86803240 @default.
- W2395297185 hasConceptScore W2395297185C87644729 @default.
- W2395297185 hasIssue "3" @default.
- W2395297185 hasLocation W23952971851 @default.
- W2395297185 hasOpenAccess W2395297185 @default.
- W2395297185 hasPrimaryLocation W23952971851 @default.
- W2395297185 hasRelatedWork W1989913538 @default.
- W2395297185 hasRelatedWork W1997890750 @default.
- W2395297185 hasRelatedWork W2035097523 @default.
- W2395297185 hasRelatedWork W2044521546 @default.
- W2395297185 hasRelatedWork W2053347593 @default.
- W2395297185 hasRelatedWork W2055687789 @default.
- W2395297185 hasRelatedWork W2070541933 @default.
- W2395297185 hasRelatedWork W2395297185 @default.
- W2395297185 hasRelatedWork W2952574653 @default.
- W2395297185 hasRelatedWork W2074929342 @default.
- W2395297185 hasVolume "18" @default.
- W2395297185 isParatext "false" @default.
- W2395297185 isRetracted "false" @default.
- W2395297185 magId "2395297185" @default.
- W2395297185 workType "article" @default.