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• W2395429571 abstract "Retrovirus-mediated mRNA transfer (RMT) combines the advantageous features of retrovirus-mediated cell targeting and entry with the controlled transfer of mRNAs. We have recently exploited this strategy for the dose-controlled transfer of recombinases and DNA transposases, avoiding cytotoxicity and potential insertional mutagenesis. Further applications can be envisaged, especially when low expression levels are sufficient to modify cell fate or function. Here we describe a step-by-step protocol for the generation of RMT vector particles, their titration and their application in a model cell line." @default.
• W2395429571 created "2016-06-24" @default.
• W2395429571 creator A5047937141 @default.
• W2395429571 creator A5054362291 @default.
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• W2395429571 date "2012-11-08" @default.
• W2395429571 modified "2023-10-06" @default.
• W2395429571 title "Retrovirus-Based mRNA Transfer for Transient Cell Manipulation" @default.
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• W2395429571 doi "https://doi.org/10.1007/978-1-62703-260-5_10" @default.
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• W2395429571 hasPublicationYear "2012" @default.
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