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- W2395646035 abstract "Objective To investigate the effects of hydroquinone (HQ) on expression of topoisomerase Ⅱα (TOPO Ⅱα ) in human bone marrow mononuclear cells,and to explore the role and possible regulatory mechanism of TOPO Ⅱα involved in toxicity of HQ to hematopoietic cells. Methods After human bone marrow mononuclear cells were exposed to 50 mol/L HQ (used the cells which were exposed to sterile distilled water as control); the activity of TOPO Ⅱ was measured by TOPO Ⅱ assay kit; the expression levels of TOPO Ⅱα mRNA and protein were detected by RT-PCR technique and Western bloting method respectively;the chromatin immunoprecipitation (ChIP) assay was carried out to study the possible mechanism of TOPO Ⅱ α expression changes. Results (1)The activity of TOPO Ⅱ was inhibited obviously; the protein and mRNA expression of TOPO Ⅱα were 0.017±0.029 and 0.610±0.128, significantly lower than that in the control with the significant difference (P 0.05); (3) TOPO Ⅱα mRNA expression decreased gradually after HQ processing, and the chemical modification (histone H4 acetylation) of TOPO Ⅱα promoter happened prior to the mRNA expression. Conclusion HQ could repress the expression of TOPO Ⅱα in human bone marrow mononuclear cells; the change of histone chemical modification plays an important role in the benzene's hematopoietic toxicity.Key words: Hydroquinones; DNA topoisomerase Ⅱα ( ATP-hydrolysing); Histones; Acetylation" @default.
- W2395646035 created "2016-06-24" @default.
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- W2395646035 date "2010-09-01" @default.
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- W2395646035 title "Effect of hydroquinone on expression of topoisomerase enzyme IIα in human bone marrow mononuclear cells" @default.
- W2395646035 doi "https://doi.org/10.3760/cma.j.issn.1001-9391.2010.09.007" @default.
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