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- W2395794024 abstract "Fresh washed platelets are commonly used as target cells for the detection of platelet reactive antibodies. The use of stored platelets would allow a faster execution of the tests and a rapid selection of phenotyped cells.Glass-adherent platelet monolayers were freeze stored. The antigen-antibody reaction between platelet antigens and platelet reactive allo- or autoantibodies was revealed by a modified antiglobulin test, using colloidal gold-labeled anti-human IgG. Immunogold staining (IGS) was compared with the platelet suspension immunofluorescence test (PSIFT) and with the solid-phase red cell adherence assay (SPRCA). The reactivity of fresh and freeze-stored platelets was compared.IGS proved to be slightly less sensitive (10%) than PSIFT and SPRCA: The results obtained with fresh and freeze-stored cells were scored identically.The immunogold staining assay on freeze-stored platelets seems to be a convenient technical approach for platelet serology." @default.
- W2395794024 created "2016-06-24" @default.
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- W2395794024 date "1992-01-01" @default.
- W2395794024 modified "2023-09-27" @default.
- W2395794024 title "Colloidal gold immunostaining for the detection of platelet reactive allo- and autoantibodies. Use on fresh and frozen cells." @default.
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