FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2395887698> ?p ?o ?g. }

• W2395887698 endingPage "43" @default.
• W2395887698 startingPage "37" @default.
• W2395887698 abstract "Endopeptidase-24.18 (E-24.18; EC 3.4.24.18) is a metallopeptidase of the astacin family and is highly expressed in kidney brush-border membranes of rodents. Rat E-24.18 consists of two disulphide-linked alpha/beta dimers [(alpha/beta)2]. In order to investigate the mechanisms of assembly and the importance of each subunit in the enzymic process, the cloned cDNAs for the rat alpha and beta subunits were transiently expressed either alone or together in COS-1 cells. Immunoblotting of cell extracts and spent culture media showed that, when expressed alone, the alpha subunit is secreted, whereas the beta subunit is membrane-bound. In alpha/beta-transfected cells, the alpha subunit remained membrane-bound, but could be released from the cell surface after papain treatment or after incubation with 10 mM dithiothreitol. Furthermore, mutants of the alpha subunit in which the putative C-terminal anchor domain was deleted could still form cell-associated alpha/beta dimers. These results are consistent with a topological model of E-24.18 in which the beta subunit is anchored in the plasma membrane and the alpha subunit is retained at the cell surface through disulphide bridge(s) with the beta subunit. Both the alpha and beta recombinant subunits expressed in COS-1 cells showed little azocasein-degrading activity. However, activity of either individual subunits of alpha/beta dimers was increased after mild trypsin digestion, suggesting that in COS-1 cells the enzymes are synthesized as zymogens. Finally, inactivation of the alpha subunit by site-directed mutagenesis of Glu-157, which is believed to play a role in catalysis, showed that both subunits participate in the enzymic activity of the heterodimer." @default.
• W2395887698 created "2016-06-24" @default.
• W2395887698 creator A5006919107 @default.
• W2395887698 creator A5043136683 @default.
• W2395887698 creator A5072500215 @default.
• W2395887698 creator A5074964938 @default.
• W2395887698 creator A5077023219 @default.
• W2395887698 creator A5082647625 @default.
• W2395887698 date "1994-05-15" @default.
• W2395887698 modified "2023-10-16" @default.
• W2395887698 title "Expression of rat endopeptidase-24.18 in COS-1 cells: membrane topology and activity" @default.
• W2395887698 cites W13914164 @default.
• W2395887698 cites W1498169126 @default.
• W2395887698 cites W1541988093 @default.
• W2395887698 cites W1560685236 @default.
• W2395887698 cites W1580027256 @default.
• W2395887698 cites W1580742681 @default.
• W2395887698 cites W1583907090 @default.
• W2395887698 cites W1607745865 @default.
• W2395887698 cites W1669475135 @default.
• W2395887698 cites W1849374011 @default.
• W2395887698 cites W1864169613 @default.
• W2395887698 cites W1952065778 @default.
• W2395887698 cites W1964062742 @default.
• W2395887698 cites W1968539554 @default.
• W2395887698 cites W1976634502 @default.
• W2395887698 cites W1978931935 @default.
• W2395887698 cites W1998395373 @default.
• W2395887698 cites W2015706285 @default.
• W2395887698 cites W2018289835 @default.
• W2395887698 cites W2024071649 @default.
• W2395887698 cites W2027536931 @default.
• W2395887698 cites W2047043554 @default.
• W2395887698 cites W2047671583 @default.
• W2395887698 cites W2049262079 @default.
• W2395887698 cites W2055403186 @default.
• W2395887698 cites W2055810510 @default.
• W2395887698 cites W2058136306 @default.
• W2395887698 cites W2060913542 @default.
• W2395887698 cites W2081370863 @default.
• W2395887698 cites W2088167128 @default.
• W2395887698 cites W2093586599 @default.
• W2395887698 cites W2097310794 @default.
• W2395887698 cites W2100837269 @default.
• W2395887698 cites W2128635872 @default.
• W2395887698 cites W2138075022 @default.
• W2395887698 cites W2233677585 @default.
• W2395887698 cites W2241265067 @default.
• W2395887698 cites W2260332405 @default.
• W2395887698 cites W2305522824 @default.
• W2395887698 cites W2342978219 @default.
• W2395887698 cites W50689081 @default.
• W2395887698 cites W57601398 @default.
• W2395887698 cites W72135613 @default.
• W2395887698 doi "https://doi.org/10.1042/bj3000037" @default.
• W2395887698 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1138119" @default.
• W2395887698 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8198548" @default.
• W2395887698 hasPublicationYear "1994" @default.
• W2395887698 type Work @default.
• W2395887698 sameAs 2395887698 @default.
• W2395887698 citedByCount "18" @default.
• W2395887698 countsByYear W23958876982013 @default.
• W2395887698 crossrefType "journal-article" @default.
• W2395887698 hasAuthorship W2395887698A5006919107 @default.
• W2395887698 hasAuthorship W2395887698A5043136683 @default.
• W2395887698 hasAuthorship W2395887698A5072500215 @default.
• W2395887698 hasAuthorship W2395887698A5074964938 @default.
• W2395887698 hasAuthorship W2395887698A5077023219 @default.
• W2395887698 hasAuthorship W2395887698A5082647625 @default.
• W2395887698 hasBestOaLocation W23958876982 @default.
• W2395887698 hasConcept C104292427 @default.
• W2395887698 hasConcept C104317684 @default.
• W2395887698 hasConcept C153911025 @default.
• W2395887698 hasConcept C159110408 @default.
• W2395887698 hasConcept C164083038 @default.
• W2395887698 hasConcept C185592680 @default.
• W2395887698 hasConcept C190472901 @default.
• W2395887698 hasConcept C199360897 @default.
• W2395887698 hasConcept C206412730 @default.
• W2395887698 hasConcept C2775944032 @default.
• W2395887698 hasConcept C2776174256 @default.
• W2395887698 hasConcept C41008148 @default.
• W2395887698 hasConcept C49453240 @default.
• W2395887698 hasConcept C54009773 @default.
• W2395887698 hasConcept C54355233 @default.
• W2395887698 hasConcept C55493867 @default.
• W2395887698 hasConcept C64943373 @default.
• W2395887698 hasConcept C71924100 @default.
• W2395887698 hasConcept C81885089 @default.
• W2395887698 hasConcept C83730767 @default.
• W2395887698 hasConcept C86803240 @default.
• W2395887698 hasConceptScore W2395887698C104292427 @default.
• W2395887698 hasConceptScore W2395887698C104317684 @default.
• W2395887698 hasConceptScore W2395887698C153911025 @default.
• W2395887698 hasConceptScore W2395887698C159110408 @default.
• W2395887698 hasConceptScore W2395887698C164083038 @default.
• W2395887698 hasConceptScore W2395887698C185592680 @default.
• W2395887698 hasConceptScore W2395887698C190472901 @default.

• next