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• W2395916326 abstract "Flatfish metamorphosis denotes the extraordinary transformation of a symmetric pelagic larva into an asymmetric benthic juvenile. Metamorphosis in vertebrates is driven by thyroid hormones (THs), but how they orchestrate the cellular, morphological and functional modifications associated with maturation to juvenile/adult states in flatfish is an enigma. Since THs act via thyroid receptors that are ligand activated transcription factors, we hypothesized that the maturation of tissues during metamorphosis should be preceded by significant modifications in the transcriptome. Targeting the unique metamorphosis of flatfish and taking advantage of the large size of Atlantic halibut (Hippoglossus hippoglossus) larvae, we determined the molecular basis of TH action using RNA sequencing.De novo assembly of sequences for larval head, skin and gastrointestinal tract (GI-tract) yielded 90,676, 65,530 and 38,426 contigs, respectively. More than 57 % of the assembled sequences were successfully annotated using a multi-step Blast approach. A unique set of biological processes and candidate genes were identified specifically associated with changes in morphology and function of the head, skin and GI-tract. Transcriptome dynamics during metamorphosis were mapped with SOLiD sequencing of whole larvae and revealed greater than 8,000 differentially expressed (DE) genes significantly (p < 0.05) up- or down-regulated in comparison with the juvenile stage. Candidate transcripts quantified by SOLiD and qPCR analysis were significantly (r = 0.843; p < 0.05) correlated. The majority (98 %) of DE genes during metamorphosis were not TH-responsive. TH-responsive transcripts clustered into 6 groups based on their expression pattern during metamorphosis and the majority of the 145 DE TH-responsive genes were down-regulated.A transcriptome resource has been generated for metamorphosing Atlantic halibut and over 8,000 DE transcripts per stage were identified. Unique sets of biological processes and candidate genes were associated with changes in the head, skin and GI-tract during metamorphosis. A small proportion of DE transcripts were TH-responsive, suggesting that they trigger gene networks, signalling cascades and transcription factors, leading to the overt changes in tissue occurring during metamorphosis." @default.
• W2395916326 created "2016-06-24" @default.
• W2395916326 creator A5007681553 @default.
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• W2395916326 date "2016-05-27" @default.
• W2395916326 modified "2023-10-11" @default.
• W2395916326 title "The transcriptome of metamorphosing flatfish" @default.
• W2395916326 cites W1520982075 @default.
• W2395916326 cites W1546664552 @default.
• W2395916326 cites W1551944231 @default.
• W2395916326 cites W174573772 @default.
• W2395916326 cites W1813355889 @default.
• W2395916326 cites W1963647662 @default.
• W2395916326 cites W1963976116 @default.
• W2395916326 cites W1964383324 @default.
• W2395916326 cites W1964765282 @default.
• W2395916326 cites W1967687840 @default.
• W2395916326 cites W1969353942 @default.
• W2395916326 cites W1970177972 @default.
• W2395916326 cites W1970483666 @default.
• W2395916326 cites W1974116356 @default.
• W2395916326 cites W1976714887 @default.
• W2395916326 cites W1980832239 @default.
• W2395916326 cites W1982066003 @default.
• W2395916326 cites W1982242935 @default.
• W2395916326 cites W1988030894 @default.
• W2395916326 cites W1988334089 @default.
• W2395916326 cites W1988568830 @default.
• W2395916326 cites W1988711764 @default.
• W2395916326 cites W1989252102 @default.
• W2395916326 cites W1989367702 @default.
• W2395916326 cites W1990540269 @default.
• W2395916326 cites W1991454487 @default.
• W2395916326 cites W1992763415 @default.
• W2395916326 cites W1993975789 @default.
• W2395916326 cites W1995933541 @default.
• W2395916326 cites W1996820841 @default.
• W2395916326 cites W1997291095 @default.
• W2395916326 cites W1999007774 @default.
• W2395916326 cites W2000006958 @default.
• W2395916326 cites W2000275880 @default.
• W2395916326 cites W2001811667 @default.
• W2395916326 cites W2003845753 @default.
• W2395916326 cites W2004287716 @default.
• W2395916326 cites W2004465685 @default.
• W2395916326 cites W2005028972 @default.
• W2395916326 cites W2011261263 @default.
• W2395916326 cites W2011366445 @default.
• W2395916326 cites W2016266442 @default.
• W2395916326 cites W2016889790 @default.
• W2395916326 cites W2017433313 @default.
• W2395916326 cites W2018134715 @default.
• W2395916326 cites W2022638217 @default.
• W2395916326 cites W2024147132 @default.
• W2395916326 cites W2025518907 @default.
• W2395916326 cites W2026274081 @default.
• W2395916326 cites W2026495855 @default.
• W2395916326 cites W2026706442 @default.
• W2395916326 cites W2027256085 @default.
• W2395916326 cites W2028422261 @default.
• W2395916326 cites W2031240949 @default.
• W2395916326 cites W2033338002 @default.
• W2395916326 cites W2035971623 @default.
• W2395916326 cites W2037910991 @default.
• W2395916326 cites W2040003075 @default.
• W2395916326 cites W2041350919 @default.
• W2395916326 cites W2043339305 @default.
• W2395916326 cites W2044833549 @default.
• W2395916326 cites W2044884536 @default.
• W2395916326 cites W2047143911 @default.
• W2395916326 cites W2051322192 @default.
• W2395916326 cites W2054695191 @default.
• W2395916326 cites W2057110190 @default.
• W2395916326 cites W2062086872 @default.
• W2395916326 cites W2062221775 @default.
• W2395916326 cites W2064489891 @default.
• W2395916326 cites W2068871850 @default.
• W2395916326 cites W2072012875 @default.
• W2395916326 cites W2073100952 @default.
• W2395916326 cites W2077753802 @default.
• W2395916326 cites W2078093008 @default.
• W2395916326 cites W2078662624 @default.
• W2395916326 cites W2080232434 @default.
• W2395916326 cites W2083361150 @default.
• W2395916326 cites W2083457899 @default.
• W2395916326 cites W2084437902 @default.
• W2395916326 cites W2089159475 @default.
• W2395916326 cites W2089164938 @default.
• W2395916326 cites W2089184462 @default.
• W2395916326 cites W2089946206 @default.
• W2395916326 cites W2091299496 @default.
• W2395916326 cites W2091546583 @default.

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