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- W2396091548 abstract "DNA sequences corresponding to gene seg- ments that code for the nonstructural protein, the matrix protein, and the hemagglutinin of influenza A virus (strain A/Udorm/72 (H3N2)) were cloned in Escherichia coli pBR 322. Initially, positive and negative cDNA strands were prepared separately by reverse transcription. The positive strands of cDNA were transcribed from genomic RNA segments by using a specific dodecamer DNA sequence as a primer; the negative strands of cDNA were transcribed from cytoplasmic viral mRNA segments by using an oligo(dT) primer. DNA duplexes corresponding in size to the virus RNA segments were then purified, inserted into the plasmid DNA, and used for transformation of E. coli. The influenza virus-specific DNA sequences isolated from recom- binant plasmid molecules were characterized by mapping re- striction enzyme cleavage sites. In addition, the orientation of cloned DNA was determined with reference to the 3' terminus of viral RNA. The negative-stranded RNA genome of influenza A virus contains eight segments that vary from 0.2 to 0.9 X 106 daltons" @default.
- W2396091548 created "2016-06-24" @default.
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- W2396091548 date "2016-01-01" @default.
- W2396091548 modified "2023-09-24" @default.
- W2396091548 title "Cloning DNA sequences from influenza viral RNA segments (cDNA strands/cleavage maps/nonstructural protein/matrix protein/hemagglutinin)" @default.
- W2396091548 hasPublicationYear "2016" @default.
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