Matches in SemOpenAlex for { <https://semopenalex.org/work/W2396188534> ?p ?o ?g. }
- W2396188534 abstract "Abstract Internal cavities are important elements in protein structure, dynamics, stability and function. Here we use NMR spectroscopy to investigate the binding of molecular oxygen (O 2 ) to cavities in a well-studied model for ligand binding, the L99A mutant of T4 lysozyme. On increasing the O 2 concentration to 8.9 mM, changes in 1 H, 15 N and 13 C chemical shifts and signal broadening were observed specifically for backbone amide and side chain methyl groups located around the two hydrophobic cavities of the protein. O 2 -induced longitudinal relaxation enhancements for amide and methyl protons could be adequately accounted for by paramagnetic dipolar relaxation. These data provide the first experimental demonstration that O 2 binds specifically to the hydrophobic and not the hydrophilic cavities, in a protein. Molecular dynamics simulations visualized the rotational and translational motions of O 2 in the cavities, as well as the binding and egress of O 2 , suggesting that the channel consisting of helices D, E, G, H and J could be the potential gateway for ligand binding to the protein. Due to strong paramagnetic relaxation effects, O 2 gas-pressure NMR measurements can detect hydrophobic cavities when populated to as little as 1% and thereby provide a general and highly sensitive method for detecting oxygen binding in proteins." @default.
- W2396188534 created "2016-06-24" @default.
- W2396188534 creator A5032503871 @default.
- W2396188534 creator A5041024225 @default.
- W2396188534 creator A5044279513 @default.
- W2396188534 creator A5061016065 @default.
- W2396188534 creator A5069162845 @default.
- W2396188534 date "2016-02-02" @default.
- W2396188534 modified "2023-10-18" @default.
- W2396188534 title "Detecting O2 binding sites in protein cavities" @default.
- W2396188534 cites W1563420247 @default.
- W2396188534 cites W1965363207 @default.
- W2396188534 cites W1975572747 @default.
- W2396188534 cites W1976499671 @default.
- W2396188534 cites W1976898303 @default.
- W2396188534 cites W1977134599 @default.
- W2396188534 cites W1981021420 @default.
- W2396188534 cites W1981687968 @default.
- W2396188534 cites W1986212429 @default.
- W2396188534 cites W1988915042 @default.
- W2396188534 cites W1991938411 @default.
- W2396188534 cites W1997529464 @default.
- W2396188534 cites W2000498167 @default.
- W2396188534 cites W2002195659 @default.
- W2396188534 cites W2006422372 @default.
- W2396188534 cites W2009222208 @default.
- W2396188534 cites W2013021618 @default.
- W2396188534 cites W2025587541 @default.
- W2396188534 cites W2027052763 @default.
- W2396188534 cites W2029667189 @default.
- W2396188534 cites W2032566522 @default.
- W2396188534 cites W2034746027 @default.
- W2396188534 cites W2035804830 @default.
- W2396188534 cites W2037913479 @default.
- W2396188534 cites W2041156123 @default.
- W2396188534 cites W2042430712 @default.
- W2396188534 cites W2042732987 @default.
- W2396188534 cites W2045280613 @default.
- W2396188534 cites W2046252238 @default.
- W2396188534 cites W2048700985 @default.
- W2396188534 cites W2050168521 @default.
- W2396188534 cites W2051381895 @default.
- W2396188534 cites W2059890035 @default.
- W2396188534 cites W2064579810 @default.
- W2396188534 cites W2066333719 @default.
- W2396188534 cites W2067174909 @default.
- W2396188534 cites W2070496025 @default.
- W2396188534 cites W2072995527 @default.
- W2396188534 cites W2073445834 @default.
- W2396188534 cites W2078574019 @default.
- W2396188534 cites W2080516426 @default.
- W2396188534 cites W2080669203 @default.
- W2396188534 cites W2081693079 @default.
- W2396188534 cites W2083385781 @default.
- W2396188534 cites W2092581523 @default.
- W2396188534 cites W2094445728 @default.
- W2396188534 cites W2096700300 @default.
- W2396188534 cites W2103722193 @default.
- W2396188534 cites W2103945336 @default.
- W2396188534 cites W2118060715 @default.
- W2396188534 cites W2128572087 @default.
- W2396188534 cites W2132717858 @default.
- W2396188534 cites W2140746956 @default.
- W2396188534 cites W2158374287 @default.
- W2396188534 cites W2169821755 @default.
- W2396188534 cites W2317796205 @default.
- W2396188534 cites W3102259202 @default.
- W2396188534 cites W3120327760 @default.
- W2396188534 cites W971104860 @default.
- W2396188534 doi "https://doi.org/10.1038/srep20534" @default.
- W2396188534 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4735865" @default.
- W2396188534 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26830762" @default.
- W2396188534 hasPublicationYear "2016" @default.
- W2396188534 type Work @default.
- W2396188534 sameAs 2396188534 @default.
- W2396188534 citedByCount "18" @default.
- W2396188534 countsByYear W23961885342016 @default.
- W2396188534 countsByYear W23961885342017 @default.
- W2396188534 countsByYear W23961885342018 @default.
- W2396188534 countsByYear W23961885342019 @default.
- W2396188534 countsByYear W23961885342022 @default.
- W2396188534 crossrefType "journal-article" @default.
- W2396188534 hasAuthorship W2396188534A5032503871 @default.
- W2396188534 hasAuthorship W2396188534A5041024225 @default.
- W2396188534 hasAuthorship W2396188534A5044279513 @default.
- W2396188534 hasAuthorship W2396188534A5061016065 @default.
- W2396188534 hasAuthorship W2396188534A5069162845 @default.
- W2396188534 hasBestOaLocation W23961885341 @default.
- W2396188534 hasConcept C107824862 @default.
- W2396188534 hasConcept C111429119 @default.
- W2396188534 hasConcept C116569031 @default.
- W2396188534 hasConcept C121332964 @default.
- W2396188534 hasConcept C124712363 @default.
- W2396188534 hasConcept C12554922 @default.
- W2396188534 hasConcept C147597530 @default.
- W2396188534 hasConcept C147789679 @default.
- W2396188534 hasConcept C15744967 @default.
- W2396188534 hasConcept C170493617 @default.
- W2396188534 hasConcept C185592680 @default.
- W2396188534 hasConcept C2776029896 @default.