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- W2396262664 abstract "Simple, rapid and sensitive methods for separation and quantification of caffeine and/or dimethylxanthines in biological fluids and tissues were developed by high performance liquid chromatography. A 0.1-ml of the serum was denatured and precipitated with 0.1 ml of acetonitrile. A 0.01-ml of the supernatant was injected into the chromatograph with a reversed-phase Zorbax ODS column and ultraviolet (UV) detector set at 273 nm and 0.01a.u.f.s. The flow rate of the mobile phase of 0.2 M acetate buffer-acetonitrile (85 : 15, v/v) was 0.5 ml/min. The limit of detection was 0.2 μg/ml for caffeine. On analyses of biological fluids, caffeine and its metabolites were extracted from 0.2 ml of the plasma or saliva by using 2.5 ml of chloroform-isopropanol solution (75 : 25, v/v). On analyses of tissues, the liver, placenta or fetal cerebrum was homogenated by saline, adjusted to 10% solution, and caffeine and its metabolites were extracted from 0.5 ml of 10% tissue homogenates by using 5.0 ml of chloroform-isopropanol solution. The reversed-phase μBondapak C18 column was used, connected with UV detector set at 280 nm and 0.01 a.u.f.s. The flow rate of mobile phase of 0.005 M acetate buffer-methanol-acetonitrile-tetrahydrofuran (92.5 : 3.0 : 2.8 : 1.7, v/v) was 1.5 ml/min. The limits of detection of caffeine and dimethylxanthines on analyses of biological fluids and tissues were 0.1-0.2 μg/ml and 1.0-2.0 μg/g wet weight, respectively. The practicability and utility of the method were demonstrated in human and rat studies." @default.
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- W2396262664 date "1985-01-01" @default.
- W2396262664 modified "2023-09-28" @default.
- W2396262664 title "Determination of Caffeine and Dimethylxanthines in Biological Fluids and Tissues by High Performance Liquid Chromatography" @default.
- W2396262664 doi "https://doi.org/10.1248/yakushi1947.105.9_848" @default.
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