Matches in SemOpenAlex for { <https://semopenalex.org/work/W2396707797> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2396707797 endingPage "62" @default.
- W2396707797 startingPage "858" @default.
- W2396707797 abstract "Respiratory syncystial virus (RSV) is the most common cause of lower respiratory infections in infants worldwide. There is no reliable vaccine or antiviral drug against RSV at present. RNA interference (RNAi) technology is a potent method to degrade expression of the cognate mRNA. In order to inhibit the replication of RSV at gene level, the effects of specific RNAi against M2-1 gene of RSV on inhibition of viral replication in cell culture system was observed in this study.RSV M2-1 gene, which plays a key role in RSV transcription, was chosen in this study and was used as target gene and recombinant plasmid pshRNA7816 targeting the mRNA of RSV M2-1 gene coding sequence was constructed. The pshRNA7816 was transfected into Hep2 cells. The effects of the pshRNA7816 on changes of cytopathogenic effect (CPE) of Hep2 cell induced by RSV infection were observed microscopically. Viral plaque forming assay and MTT assay were used to detect the viral titer change and protective function of the pshRNA7816 on RSV infected Hep2 cell.The recombinant RNAi plasmid pshRNA7816 which targets the mRNA of RSV M2-1 gene was successfully constructed. The pshRNA7816 significantly reduced CPE of RSV infected Hep2 cells, reduced the viral titer of RSV in the cells (P < 0.001). The pshRNA7816 raised the survival rate of RSV infected Hep2 cells (P < 0.001). Non-specific pshRNA plasmid did not show anti-RSV effects (P > 0.05).The recombinant pshRNA7816 plasmid which targeted the mRNA of RSV M2-1 gene showed a significant and specific anti-RSV effect." @default.
- W2396707797 created "2016-06-24" @default.
- W2396707797 creator A5019961292 @default.
- W2396707797 creator A5031370540 @default.
- W2396707797 creator A5051441217 @default.
- W2396707797 creator A5079447763 @default.
- W2396707797 creator A5082675176 @default.
- W2396707797 creator A5088179908 @default.
- W2396707797 date "2005-11-01" @default.
- W2396707797 modified "2023-09-27" @default.
- W2396707797 title "[Construction and effect of the recombinant pshRNA plasmid against respiratory syncystial virus M2-1 gene]." @default.
- W2396707797 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16316538" @default.
- W2396707797 hasPublicationYear "2005" @default.
- W2396707797 type Work @default.
- W2396707797 sameAs 2396707797 @default.
- W2396707797 citedByCount "0" @default.
- W2396707797 crossrefType "journal-article" @default.
- W2396707797 hasAuthorship W2396707797A5019961292 @default.
- W2396707797 hasAuthorship W2396707797A5031370540 @default.
- W2396707797 hasAuthorship W2396707797A5051441217 @default.
- W2396707797 hasAuthorship W2396707797A5079447763 @default.
- W2396707797 hasAuthorship W2396707797A5082675176 @default.
- W2396707797 hasAuthorship W2396707797A5088179908 @default.
- W2396707797 hasConcept C104317684 @default.
- W2396707797 hasConcept C140704245 @default.
- W2396707797 hasConcept C153911025 @default.
- W2396707797 hasConcept C159047783 @default.
- W2396707797 hasConcept C166703698 @default.
- W2396707797 hasConcept C190232843 @default.
- W2396707797 hasConcept C22744801 @default.
- W2396707797 hasConcept C2522874641 @default.
- W2396707797 hasConcept C32470452 @default.
- W2396707797 hasConcept C32611913 @default.
- W2396707797 hasConcept C40767141 @default.
- W2396707797 hasConcept C54009773 @default.
- W2396707797 hasConcept C54355233 @default.
- W2396707797 hasConcept C67705224 @default.
- W2396707797 hasConcept C81885089 @default.
- W2396707797 hasConcept C86803240 @default.
- W2396707797 hasConceptScore W2396707797C104317684 @default.
- W2396707797 hasConceptScore W2396707797C140704245 @default.
- W2396707797 hasConceptScore W2396707797C153911025 @default.
- W2396707797 hasConceptScore W2396707797C159047783 @default.
- W2396707797 hasConceptScore W2396707797C166703698 @default.
- W2396707797 hasConceptScore W2396707797C190232843 @default.
- W2396707797 hasConceptScore W2396707797C22744801 @default.
- W2396707797 hasConceptScore W2396707797C2522874641 @default.
- W2396707797 hasConceptScore W2396707797C32470452 @default.
- W2396707797 hasConceptScore W2396707797C32611913 @default.
- W2396707797 hasConceptScore W2396707797C40767141 @default.
- W2396707797 hasConceptScore W2396707797C54009773 @default.
- W2396707797 hasConceptScore W2396707797C54355233 @default.
- W2396707797 hasConceptScore W2396707797C67705224 @default.
- W2396707797 hasConceptScore W2396707797C81885089 @default.
- W2396707797 hasConceptScore W2396707797C86803240 @default.
- W2396707797 hasIssue "11" @default.
- W2396707797 hasLocation W23967077971 @default.
- W2396707797 hasOpenAccess W2396707797 @default.
- W2396707797 hasPrimaryLocation W23967077971 @default.
- W2396707797 hasRelatedWork W2015929165 @default.
- W2396707797 hasRelatedWork W2018382734 @default.
- W2396707797 hasRelatedWork W2084501800 @default.
- W2396707797 hasRelatedWork W2107127801 @default.
- W2396707797 hasRelatedWork W2112225216 @default.
- W2396707797 hasRelatedWork W2125543081 @default.
- W2396707797 hasRelatedWork W2136978151 @default.
- W2396707797 hasRelatedWork W2142290619 @default.
- W2396707797 hasRelatedWork W2154981459 @default.
- W2396707797 hasRelatedWork W2361768472 @default.
- W2396707797 hasRelatedWork W2379677341 @default.
- W2396707797 hasRelatedWork W2506822174 @default.
- W2396707797 hasRelatedWork W2736742531 @default.
- W2396707797 hasRelatedWork W2783357101 @default.
- W2396707797 hasRelatedWork W2809490425 @default.
- W2396707797 hasRelatedWork W3127651925 @default.
- W2396707797 hasRelatedWork W3211281820 @default.
- W2396707797 hasRelatedWork W2853577961 @default.
- W2396707797 hasRelatedWork W3142734494 @default.
- W2396707797 hasRelatedWork W3149247880 @default.
- W2396707797 hasVolume "43" @default.
- W2396707797 isParatext "false" @default.
- W2396707797 isRetracted "false" @default.
- W2396707797 magId "2396707797" @default.
- W2396707797 workType "article" @default.