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• W2396721008 abstract "The quantification of global DNA methylation has been established in epigenetic screening. As more practicable alternatives to the HPLC-based gold standard, the methylation analysis of CpG islands in repeatable elements (LINE-1) and the luminometric methylation assay (LUMA) of overall 5-methylcytosine content in “CCGG” recognition sites are most widely used. Both methods are applied as virtually equivalent, despite the hints that their results only partly agree. This triggered the present agreement assessments. Three different human cell types (cultured MCF7 and SHSY5Y cell lines treated with different chemical modulators of DNA methylation and whole blood drawn from pain patients and healthy volunteers) were submitted to the global DNA methylation assays employing LINE-1 or LUMA-based pyrosequencing measurements. The agreement between the two bioassays was assessed using generally accepted approaches to the statistics for laboratory method comparison studies. Although global DNA methylation levels measured by the two methods correlated, five different lines of statistical evidence consistently rejected the assumption of complete agreement. Specifically, a bias was observed between the two methods. In addition, both the magnitude and direction of bias were tissue-dependent. Interassay differences could be grouped based on Bayesian statistics, and these groups allowed in turn to re-identify the originating tissue. Although providing partly correlated measurements of DNA methylation, interchangeability of the quantitative results obtained with LINE-1 and LUMA was jeopardized by a consistent bias between the results. Moreover, the present analyses strongly indicate a tissue specificity of the differences between the two methods." @default.
• W2396721008 created "2016-06-24" @default.
• W2396721008 creator A5017400006 @default.
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• W2396721008 date "2016-05-23" @default.
• W2396721008 modified "2023-10-11" @default.
• W2396721008 title "Disagreement between two common biomarkers of global DNA methylation" @default.
• W2396721008 cites W101230951 @default.
• W2396721008 cites W1531710418 @default.
• W2396721008 cites W1552546752 @default.
• W2396721008 cites W1698395555 @default.
• W2396721008 cites W1959835345 @default.
• W2396721008 cites W1964394060 @default.
• W2396721008 cites W1967133987 @default.
• W2396721008 cites W1967841068 @default.
• W2396721008 cites W1971020922 @default.
• W2396721008 cites W1971617142 @default.
• W2396721008 cites W1974971448 @default.
• W2396721008 cites W1983307705 @default.
• W2396721008 cites W1988406995 @default.
• W2396721008 cites W1992393512 @default.
• W2396721008 cites W1995740954 @default.
• W2396721008 cites W2013143444 @default.
• W2396721008 cites W2013154874 @default.
• W2396721008 cites W2015521887 @default.
• W2396721008 cites W2015795623 @default.
• W2396721008 cites W2016545990 @default.
• W2396721008 cites W2022976083 @default.
• W2396721008 cites W2023614829 @default.
• W2396721008 cites W2024819383 @default.
• W2396721008 cites W2026516961 @default.
• W2396721008 cites W2028423120 @default.
• W2396721008 cites W2033732445 @default.
• W2396721008 cites W2035582624 @default.
• W2396721008 cites W2037557484 @default.
• W2396721008 cites W2041013779 @default.
• W2396721008 cites W2044616072 @default.
• W2396721008 cites W2051274365 @default.
• W2396721008 cites W2051655675 @default.
• W2396721008 cites W2052355316 @default.
• W2396721008 cites W2054692302 @default.
• W2396721008 cites W2054983569 @default.
• W2396721008 cites W2059562857 @default.
• W2396721008 cites W2062689923 @default.
• W2396721008 cites W2063594683 @default.
• W2396721008 cites W2064452286 @default.
• W2396721008 cites W2064498971 @default.
• W2396721008 cites W2065274751 @default.
• W2396721008 cites W2069215536 @default.
• W2396721008 cites W2069386104 @default.
• W2396721008 cites W2072995929 @default.
• W2396721008 cites W2077359776 @default.
• W2396721008 cites W2078483536 @default.
• W2396721008 cites W2079822589 @default.
• W2396721008 cites W2079898210 @default.
• W2396721008 cites W2080351920 @default.
• W2396721008 cites W2085953797 @default.
• W2396721008 cites W2090253646 @default.
• W2396721008 cites W2101709592 @default.
• W2396721008 cites W2102732501 @default.
• W2396721008 cites W2106401558 @default.
• W2396721008 cites W2106833395 @default.
• W2396721008 cites W2109881276 @default.
• W2396721008 cites W2114127218 @default.
• W2396721008 cites W2114161855 @default.
• W2396721008 cites W2115012618 @default.
• W2396721008 cites W2123032740 @default.
• W2396721008 cites W2127367027 @default.
• W2396721008 cites W2128149351 @default.
• W2396721008 cites W2130189740 @default.
• W2396721008 cites W2131444098 @default.
• W2396721008 cites W2135471644 @default.
• W2396721008 cites W2141124547 @default.
• W2396721008 cites W2145680370 @default.
• W2396721008 cites W2148033621 @default.
• W2396721008 cites W2153250619 @default.
• W2396721008 cites W2155701198 @default.
• W2396721008 cites W2160393778 @default.
• W2396721008 cites W2161589982 @default.
• W2396721008 cites W2162292699 @default.
• W2396721008 cites W2167617927 @default.
• W2396721008 cites W2181831046 @default.
• W2396721008 cites W2196092246 @default.
• W2396721008 cites W2221018455 @default.
• W2396721008 cites W2254690077 @default.
• W2396721008 cites W2293224832 @default.
• W2396721008 cites W2499178474 @default.
• W2396721008 cites W4243177274 @default.
• W2396721008 cites W4252684946 @default.
• W2396721008 doi "https://doi.org/10.1186/s13148-016-0227-0" @default.
• W2396721008 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4877994" @default.
• W2396721008 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27222668" @default.
• W2396721008 hasPublicationYear "2016" @default.
• W2396721008 type Work @default.
• W2396721008 sameAs 2396721008 @default.

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