Matches in SemOpenAlex for { <https://semopenalex.org/work/W2396750853> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2396750853 endingPage "F93" @default.
- W2396750853 startingPage "F88" @default.
- W2396750853 abstract "The relationship between Ca2+ and intracellular pH (pHi) was studied in the rabbit collecting duct from the inner stripe of the outer medulla (OMCDi). When bath Ca2+ was lowered [1 mM Ca2+ plus 2 mM ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA)] intracellular Ca2+ concentration ([Ca2+]i) decreased by 38.3 +/- 6.2% from 265 +/- 25 to 165 +/- 25 nm (n = 4). Lowering bath Ca2+ produced a biphasic response in pHi, transient acidification (from 6.93 +/- 0.06 to 6.80 +/- 0.06) followed by a return of pHi to levels slightly higher than control (pHi = 6.97 +/- 0.06). The acidification was not blocked by bath amiloride or 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) or lumen N-ethylmaleimide (NEM). However, bath amiloride significantly reduced the initial rate of pHi recovery after acidification (0.70 pH U/min in control vs. 0.39 pH U/min with amiloride). Lanthanum, a Ca2+ pump inhibitor, completely inhibited the acidification. Moreover, lowering bath temperature to 20 degrees C also inhibited the acidification. Acidification was blocked when the bath pH was raised to 8.0, whereas lowering bath Ca2+ markedly acidified cells when bath pH was lowered to 6.0 (delta pHi = 0.28 +/- 0.01). We also measured the transepithelial (Vt) and basolateral (Vbl) membrane voltages. Lowering bath Ca2+ depolarized Vt and Vbl by 12.7 +/- 4.9 and 6.0 +/- 2.0 mV, respectively. In summary, 1) lowering bath Ca2+ caused a biphasic response in pHi of OMCDi cells, and 2) the acidification phase is due to H+ influx associated with the Ca2+ pump." @default.
- W2396750853 created "2016-06-24" @default.
- W2396750853 creator A5011433645 @default.
- W2396750853 creator A5024921811 @default.
- W2396750853 creator A5071722069 @default.
- W2396750853 date "1993-01-01" @default.
- W2396750853 modified "2023-09-23" @default.
- W2396750853 title "Evidence for basolateral membrane Ca2+/H+ exchange in outer medullary collecting duct" @default.
- W2396750853 doi "https://doi.org/10.1152/ajprenal.1993.264.1.f88" @default.
- W2396750853 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8381606" @default.
- W2396750853 hasPublicationYear "1993" @default.
- W2396750853 type Work @default.
- W2396750853 sameAs 2396750853 @default.
- W2396750853 citedByCount "1" @default.
- W2396750853 crossrefType "journal-article" @default.
- W2396750853 hasAuthorship W2396750853A5011433645 @default.
- W2396750853 hasAuthorship W2396750853A5024921811 @default.
- W2396750853 hasAuthorship W2396750853A5071722069 @default.
- W2396750853 hasConcept C12554922 @default.
- W2396750853 hasConcept C134897140 @default.
- W2396750853 hasConcept C13965031 @default.
- W2396750853 hasConcept C178790620 @default.
- W2396750853 hasConcept C185592680 @default.
- W2396750853 hasConcept C2777658833 @default.
- W2396750853 hasConcept C2779533339 @default.
- W2396750853 hasConcept C2779608910 @default.
- W2396750853 hasConcept C39587587 @default.
- W2396750853 hasConcept C41625074 @default.
- W2396750853 hasConcept C519063684 @default.
- W2396750853 hasConcept C537181965 @default.
- W2396750853 hasConcept C55493867 @default.
- W2396750853 hasConcept C57181701 @default.
- W2396750853 hasConcept C79879829 @default.
- W2396750853 hasConcept C86803240 @default.
- W2396750853 hasConcept C99975950 @default.
- W2396750853 hasConceptScore W2396750853C12554922 @default.
- W2396750853 hasConceptScore W2396750853C134897140 @default.
- W2396750853 hasConceptScore W2396750853C13965031 @default.
- W2396750853 hasConceptScore W2396750853C178790620 @default.
- W2396750853 hasConceptScore W2396750853C185592680 @default.
- W2396750853 hasConceptScore W2396750853C2777658833 @default.
- W2396750853 hasConceptScore W2396750853C2779533339 @default.
- W2396750853 hasConceptScore W2396750853C2779608910 @default.
- W2396750853 hasConceptScore W2396750853C39587587 @default.
- W2396750853 hasConceptScore W2396750853C41625074 @default.
- W2396750853 hasConceptScore W2396750853C519063684 @default.
- W2396750853 hasConceptScore W2396750853C537181965 @default.
- W2396750853 hasConceptScore W2396750853C55493867 @default.
- W2396750853 hasConceptScore W2396750853C57181701 @default.
- W2396750853 hasConceptScore W2396750853C79879829 @default.
- W2396750853 hasConceptScore W2396750853C86803240 @default.
- W2396750853 hasConceptScore W2396750853C99975950 @default.
- W2396750853 hasIssue "1" @default.
- W2396750853 hasLocation W23967508531 @default.
- W2396750853 hasLocation W23967508532 @default.
- W2396750853 hasOpenAccess W2396750853 @default.
- W2396750853 hasPrimaryLocation W23967508531 @default.
- W2396750853 hasRelatedWork W1975246518 @default.
- W2396750853 hasRelatedWork W2023559348 @default.
- W2396750853 hasRelatedWork W2168217761 @default.
- W2396750853 hasRelatedWork W2343025792 @default.
- W2396750853 hasRelatedWork W2396750853 @default.
- W2396750853 hasRelatedWork W2410539292 @default.
- W2396750853 hasRelatedWork W2415448705 @default.
- W2396750853 hasRelatedWork W2416966341 @default.
- W2396750853 hasRelatedWork W2472287942 @default.
- W2396750853 hasRelatedWork W2035310155 @default.
- W2396750853 hasVolume "264" @default.
- W2396750853 isParatext "false" @default.
- W2396750853 isRetracted "false" @default.
- W2396750853 magId "2396750853" @default.
- W2396750853 workType "article" @default.