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- W2396835334 abstract "Carboxymethyloxime of aflatoxin B1 (AFB1) was synthesized by refluxing AF B1 with carboxymethoxylamine hemihydrochloride for 4 hours in the presence of pyridine. AF B1-BSA (antigen) was made successfully by coupling AF B1-oxime with bovine serum albumin (BSA) in the presence of a water soluble carbodiimide. The rabbits were immunized with the artificial immunogen which had been purified by dialysis. The titers of antisera were determined by indirect enzyme-linked immunosorbent assay (ELISA) which was developed by ourselves. The highest titer was 1:100,000." @default.
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- W2396835334 date "1992-09-01" @default.
- W2396835334 modified "2023-09-27" @default.
- W2396835334 title "[Preparation of antigen and antibody against aflatoxin B1]." @default.
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