Matches in SemOpenAlex for { <https://semopenalex.org/work/W2396982015> ?p ?o ?g. }
- W2396982015 abstract "Abstract Genome editing using the CRISPR/Cas9 system can be used to modify plant genomes, however, improvements in specificity and applicability are still needed in order for the editing technique to be useful in various plant species. Here, using genome editing mediated by a truncated gRNA (tru-gRNA)/Cas9 combination, we generated new alleles for OST2 , a proton pump in Arabidopsis, with no off-target effects. By following expression of Cas9 and the tru-gRNAs, newly generated mutations in CRIPSR/Cas9 transgenic plants were detected with high average mutation rates of up to 32.8% and no off-target effects using constitutive promoter. Reducing nuclear localization signals in Cas9 decreased the mutation rate. In contrast, tru-gRNA Cas9 cassettes driven by meristematic- and reproductive-tissue-specific promoters increased the heritable mutation rate in Arabidopsis, showing that high expression in the germ line can produce bi-allelic mutations. Finally, the new mutant alleles obtained for OST2 exhibited altered stomatal closing in response to environmental conditions. These results suggest further applications in molecular breeding to improve plant function using optimized plant CRISPR/Cas9 systems." @default.
- W2396982015 created "2016-06-24" @default.
- W2396982015 creator A5005357481 @default.
- W2396982015 creator A5027983969 @default.
- W2396982015 creator A5037433690 @default.
- W2396982015 creator A5038187064 @default.
- W2396982015 creator A5054315487 @default.
- W2396982015 creator A5056066629 @default.
- W2396982015 creator A5075181929 @default.
- W2396982015 date "2016-05-26" @default.
- W2396982015 modified "2023-10-16" @default.
- W2396982015 title "Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants" @default.
- W2396982015 cites W1887675732 @default.
- W2396982015 cites W1937054661 @default.
- W2396982015 cites W1968342623 @default.
- W2396982015 cites W1980288203 @default.
- W2396982015 cites W1990372437 @default.
- W2396982015 cites W1990379499 @default.
- W2396982015 cites W1998679905 @default.
- W2396982015 cites W2000768580 @default.
- W2396982015 cites W2001845502 @default.
- W2396982015 cites W2002712030 @default.
- W2396982015 cites W2003171404 @default.
- W2396982015 cites W2011164920 @default.
- W2396982015 cites W2013148539 @default.
- W2396982015 cites W2017746652 @default.
- W2396982015 cites W2019670881 @default.
- W2396982015 cites W2021341670 @default.
- W2396982015 cites W2021767455 @default.
- W2396982015 cites W2026520402 @default.
- W2396982015 cites W2036131768 @default.
- W2396982015 cites W2040665850 @default.
- W2396982015 cites W2041246911 @default.
- W2396982015 cites W2045435533 @default.
- W2396982015 cites W2046639072 @default.
- W2396982015 cites W2047312008 @default.
- W2396982015 cites W2064815984 @default.
- W2396982015 cites W2073412341 @default.
- W2396982015 cites W2075049721 @default.
- W2396982015 cites W2076555418 @default.
- W2396982015 cites W2081261052 @default.
- W2396982015 cites W2084697631 @default.
- W2396982015 cites W2085633386 @default.
- W2396982015 cites W2090303381 @default.
- W2396982015 cites W2108038398 @default.
- W2396982015 cites W2110651606 @default.
- W2396982015 cites W2113385887 @default.
- W2396982015 cites W2115189270 @default.
- W2396982015 cites W2115420193 @default.
- W2396982015 cites W2117131162 @default.
- W2396982015 cites W2123205135 @default.
- W2396982015 cites W2123872688 @default.
- W2396982015 cites W2126325519 @default.
- W2396982015 cites W2127313385 @default.
- W2396982015 cites W2131085040 @default.
- W2396982015 cites W2136656609 @default.
- W2396982015 cites W2146387619 @default.
- W2396982015 cites W2149402050 @default.
- W2396982015 cites W2152339377 @default.
- W2396982015 cites W2152700648 @default.
- W2396982015 cites W2153344788 @default.
- W2396982015 cites W2155194596 @default.
- W2396982015 cites W2157451936 @default.
- W2396982015 cites W2166363131 @default.
- W2396982015 cites W2167380395 @default.
- W2396982015 cites W2196121581 @default.
- W2396982015 cites W4236711181 @default.
- W2396982015 cites W994421439 @default.
- W2396982015 doi "https://doi.org/10.1038/srep26685" @default.
- W2396982015 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4880914" @default.
- W2396982015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27226176" @default.
- W2396982015 hasPublicationYear "2016" @default.
- W2396982015 type Work @default.
- W2396982015 sameAs 2396982015 @default.
- W2396982015 citedByCount "240" @default.
- W2396982015 countsByYear W23969820152016 @default.
- W2396982015 countsByYear W23969820152017 @default.
- W2396982015 countsByYear W23969820152018 @default.
- W2396982015 countsByYear W23969820152019 @default.
- W2396982015 countsByYear W23969820152020 @default.
- W2396982015 countsByYear W23969820152021 @default.
- W2396982015 countsByYear W23969820152022 @default.
- W2396982015 countsByYear W23969820152023 @default.
- W2396982015 crossrefType "journal-article" @default.
- W2396982015 hasAuthorship W2396982015A5005357481 @default.
- W2396982015 hasAuthorship W2396982015A5027983969 @default.
- W2396982015 hasAuthorship W2396982015A5037433690 @default.
- W2396982015 hasAuthorship W2396982015A5038187064 @default.
- W2396982015 hasAuthorship W2396982015A5054315487 @default.
- W2396982015 hasAuthorship W2396982015A5056066629 @default.
- W2396982015 hasAuthorship W2396982015A5075181929 @default.
- W2396982015 hasBestOaLocation W23969820151 @default.
- W2396982015 hasConcept C104317684 @default.
- W2396982015 hasConcept C132455925 @default.
- W2396982015 hasConcept C141231307 @default.
- W2396982015 hasConcept C143065580 @default.
- W2396982015 hasConcept C144501496 @default.
- W2396982015 hasConcept C2779491563 @default.
- W2396982015 hasConcept C501734568 @default.
- W2396982015 hasConcept C54355233 @default.