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- W2397074686 abstract "The -glucosidase gene from Streptomyces coelicolor A3(2) was cloned and expressed in Escherichia coli. The ORF consisted of 1377 nucleotides encoding 51 kDa in a predicted molecular weight. Effects of pH indicated that the -glucosidase showed similar activity using -pNPG(-nitrophenyl--D-glucopyranoside), -pNPG(-nitrophenyl--D-glucopyranoside), and -pNPF(-nitrophenyl--D-fucopyranoside) at range of pH 3 to 10, and high activity using -pNPGA (-nitrophenyl--D-galactopyranoside) from pH 5 to 10, especially, 3.3 times higher activity at pH 9. Effects of temperature indicated that the -glucosidase showed low activity using -pNPG, -pNPG, and -pNPF from to , and increased activity using -pNPGA from to , 1.8 times higher activity at than at . According to activity determination of other substrates, the enzyme was active on daidzin, genistin, and glycitin, inactive on esculin and apigenin-7-glucose. The EDTA and DTT as reducing agents inhibited -glucosidase activity, but SDS and mercaptoethanol did not inhibit. Monovalent or divalent metal ions such as , , KCl, and did not inhibited -glucosidase activity. and NaCl showed low inhibition, and inhibited 3.3 times higher than control." @default.
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- W2397074686 date "2009-01-01" @default.
- W2397074686 modified "2023-09-22" @default.
- W2397074686 title "Cloning of $beta$-Glucosidase Gene from Streptomyces coelicolor A3(2) and Characterization of the Recombinant $beta$-Glucosidase Expressed in Escherichia coli" @default.
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