Matches in SemOpenAlex for { <https://semopenalex.org/work/W2397142423> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2397142423 endingPage "10" @default.
- W2397142423 startingPage "7" @default.
- W2397142423 abstract "Objective To construct the recombinant adeno-associated virus serotype 8（AAV8）of mouse TFEB gene and to observe its abi Iity to infect 293 cells. Methods The pUC57-m TFEB and the vector plasmid was digested respectively,connected and transformed to the recombinant plasmid pAAV-CMV-mTFEB. The recombinant plasmid was identified by po Iymerasechain reaction（PCR）and sequencing. The correct recombinant plasmid was packaged within AAV8. Then the recombinant adeno-associated virus of mouse TFEB gene was transfected into 293 ce IIs. After transfection,the expression level of target gene was analyzed by Western Blot. Results The success of TFEB gene inserted into the Adeno-associated virus vector was confirmed by restriction endonucIease and squencing. The titer of the recombinant adeno-associated virus was 4.65×10 12 vg/m L. The expression of TFEB in experiment cells has increased over six-fold than that in the control（P<0.01）. Conclusion The rAAV2/8-CMV-mTFEB was successfu IIy constructed and pakaged. Furthermore,it could transfected and expressed in cells effectively." @default.
- W2397142423 created "2016-06-24" @default.
- W2397142423 creator A5000238995 @default.
- W2397142423 creator A5038261237 @default.
- W2397142423 creator A5048649643 @default.
- W2397142423 date "2016-01-01" @default.
- W2397142423 modified "2023-09-28" @default.
- W2397142423 title "Construction and identification of mouse TFEB gene recombinant adeno-associated virus" @default.
- W2397142423 hasPublicationYear "2016" @default.
- W2397142423 type Work @default.
- W2397142423 sameAs 2397142423 @default.
- W2397142423 citedByCount "0" @default.
- W2397142423 crossrefType "journal-article" @default.
- W2397142423 hasAuthorship W2397142423A5000238995 @default.
- W2397142423 hasAuthorship W2397142423A5038261237 @default.
- W2397142423 hasAuthorship W2397142423A5048649643 @default.
- W2397142423 hasConcept C104317684 @default.
- W2397142423 hasConcept C131934819 @default.
- W2397142423 hasConcept C132647116 @default.
- W2397142423 hasConcept C153911025 @default.
- W2397142423 hasConcept C159047783 @default.
- W2397142423 hasConcept C22744801 @default.
- W2397142423 hasConcept C2522874641 @default.
- W2397142423 hasConcept C2778107364 @default.
- W2397142423 hasConcept C32470452 @default.
- W2397142423 hasConcept C39060996 @default.
- W2397142423 hasConcept C40767141 @default.
- W2397142423 hasConcept C54009773 @default.
- W2397142423 hasConcept C54355233 @default.
- W2397142423 hasConcept C86803240 @default.
- W2397142423 hasConcept C92087593 @default.
- W2397142423 hasConceptScore W2397142423C104317684 @default.
- W2397142423 hasConceptScore W2397142423C131934819 @default.
- W2397142423 hasConceptScore W2397142423C132647116 @default.
- W2397142423 hasConceptScore W2397142423C153911025 @default.
- W2397142423 hasConceptScore W2397142423C159047783 @default.
- W2397142423 hasConceptScore W2397142423C22744801 @default.
- W2397142423 hasConceptScore W2397142423C2522874641 @default.
- W2397142423 hasConceptScore W2397142423C2778107364 @default.
- W2397142423 hasConceptScore W2397142423C32470452 @default.
- W2397142423 hasConceptScore W2397142423C39060996 @default.
- W2397142423 hasConceptScore W2397142423C40767141 @default.
- W2397142423 hasConceptScore W2397142423C54009773 @default.
- W2397142423 hasConceptScore W2397142423C54355233 @default.
- W2397142423 hasConceptScore W2397142423C86803240 @default.
- W2397142423 hasConceptScore W2397142423C92087593 @default.
- W2397142423 hasIssue "1" @default.
- W2397142423 hasLocation W23971424231 @default.
- W2397142423 hasOpenAccess W2397142423 @default.
- W2397142423 hasPrimaryLocation W23971424231 @default.
- W2397142423 hasRelatedWork W1983074066 @default.
- W2397142423 hasRelatedWork W2351233171 @default.
- W2397142423 hasRelatedWork W2351625714 @default.
- W2397142423 hasRelatedWork W2353086735 @default.
- W2397142423 hasRelatedWork W2354011035 @default.
- W2397142423 hasRelatedWork W2356324463 @default.
- W2397142423 hasRelatedWork W2356625414 @default.
- W2397142423 hasRelatedWork W2357064473 @default.
- W2397142423 hasRelatedWork W2357736139 @default.
- W2397142423 hasRelatedWork W2367859800 @default.
- W2397142423 hasRelatedWork W2368066453 @default.
- W2397142423 hasRelatedWork W2374233850 @default.
- W2397142423 hasRelatedWork W2378253534 @default.
- W2397142423 hasRelatedWork W2391479029 @default.
- W2397142423 hasRelatedWork W2408358546 @default.
- W2397142423 hasRelatedWork W2413847679 @default.
- W2397142423 hasRelatedWork W2420122227 @default.
- W2397142423 hasRelatedWork W3030814607 @default.
- W2397142423 hasRelatedWork W3032635393 @default.
- W2397142423 hasRelatedWork W2188458189 @default.
- W2397142423 isParatext "false" @default.
- W2397142423 isRetracted "false" @default.
- W2397142423 magId "2397142423" @default.
- W2397142423 workType "article" @default.