Matches in SemOpenAlex for { <https://semopenalex.org/work/W2397360055> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2397360055 abstract "We have solved the crystal structure of a predicted fructose-specific enzyme IIB(fruc) from Escherichia coli (EcEIIB(fruc)) involved in the phosphoenolpyruvate-carbohydrate phosphotransferase system transferring carbohydrates across the cytoplasmic membrane. EcEIIB(fruc) belongs to a sequence family with more than 5,000 sequence homologues with 25-99% amino-acid sequence identity. It reveals a conventional Rossmann-like α-β-α sandwich fold with a unique β-sheet topology. Its C-terminus is longer than its closest relatives and forms an additional β-strand whereas the shorter C-terminus is random coil in the relatives. Interestingly, its core structure is similar to that of enzyme IIB(cellobiose) from E. coli (EcIIB(cel)) transferring a phosphate moiety. In the active site of the closest EcEIIB(fruc) homologues, a unique motif CXXGXAHT comprising a P-loop like architecture including a histidine residue is found. The conserved cysteine on this loop may be deprotonated to act as a nucleophile similar to that of EcIIB(cel). The conserved histidine residue is presumed to bind the negatively charged phosphate. Therefore, we propose that the catalytic mechanism of EcEIIB(fruc) is similar to that of EcIIB(cel) transferring phosphoryl moiety to a specific carbohydrate." @default.
- W2397360055 created "2016-06-24" @default.
- W2397360055 creator A5001352482 @default.
- W2397360055 creator A5009715789 @default.
- W2397360055 creator A5034124080 @default.
- W2397360055 creator A5038483388 @default.
- W2397360055 creator A5047082140 @default.
- W2397360055 creator A5052434732 @default.
- W2397360055 creator A5070875054 @default.
- W2397360055 date "2016-05-24" @default.
- W2397360055 modified "2023-09-26" @default.
- W2397360055 title "Crystal Structure of Hypothetical Fructose-Specific EIIB from Escherichia coli" @default.
- W2397360055 cites W1973180388 @default.
- W2397360055 cites W2001365149 @default.
- W2397360055 cites W2004377868 @default.
- W2397360055 cites W2014676266 @default.
- W2397360055 cites W2023728645 @default.
- W2397360055 cites W2030517878 @default.
- W2397360055 cites W2063474262 @default.
- W2397360055 cites W2070048660 @default.
- W2397360055 cites W2071320074 @default.
- W2397360055 cites W2083042788 @default.
- W2397360055 cites W2088587825 @default.
- W2397360055 cites W2108620037 @default.
- W2397360055 cites W2120772351 @default.
- W2397360055 cites W2138956739 @default.
- W2397360055 cites W2153544371 @default.
- W2397360055 cites W2154197653 @default.
- W2397360055 cites W2180229411 @default.
- W2397360055 cites W2327790154 @default.
- W2397360055 doi "https://doi.org/10.14348/molcells.2016.0055" @default.
- W2397360055 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4916401" @default.
- W2397360055 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27215198" @default.
- W2397360055 hasPublicationYear "2016" @default.
- W2397360055 type Work @default.
- W2397360055 sameAs 2397360055 @default.
- W2397360055 citedByCount "2" @default.
- W2397360055 countsByYear W23973600552019 @default.
- W2397360055 crossrefType "journal-article" @default.
- W2397360055 hasAuthorship W2397360055A5001352482 @default.
- W2397360055 hasAuthorship W2397360055A5009715789 @default.
- W2397360055 hasAuthorship W2397360055A5034124080 @default.
- W2397360055 hasAuthorship W2397360055A5038483388 @default.
- W2397360055 hasAuthorship W2397360055A5047082140 @default.
- W2397360055 hasAuthorship W2397360055A5052434732 @default.
- W2397360055 hasAuthorship W2397360055A5070875054 @default.
- W2397360055 hasBestOaLocation W23973600551 @default.
- W2397360055 hasConcept C104317684 @default.
- W2397360055 hasConcept C143937172 @default.
- W2397360055 hasConcept C167625842 @default.
- W2397360055 hasConcept C181199279 @default.
- W2397360055 hasConcept C185592680 @default.
- W2397360055 hasConcept C196032511 @default.
- W2397360055 hasConcept C2776568683 @default.
- W2397360055 hasConcept C2778460671 @default.
- W2397360055 hasConcept C2779201268 @default.
- W2397360055 hasConcept C2781338088 @default.
- W2397360055 hasConcept C41183919 @default.
- W2397360055 hasConcept C45656491 @default.
- W2397360055 hasConcept C547475151 @default.
- W2397360055 hasConcept C55493867 @default.
- W2397360055 hasConcept C71240020 @default.
- W2397360055 hasConceptScore W2397360055C104317684 @default.
- W2397360055 hasConceptScore W2397360055C143937172 @default.
- W2397360055 hasConceptScore W2397360055C167625842 @default.
- W2397360055 hasConceptScore W2397360055C181199279 @default.
- W2397360055 hasConceptScore W2397360055C185592680 @default.
- W2397360055 hasConceptScore W2397360055C196032511 @default.
- W2397360055 hasConceptScore W2397360055C2776568683 @default.
- W2397360055 hasConceptScore W2397360055C2778460671 @default.
- W2397360055 hasConceptScore W2397360055C2779201268 @default.
- W2397360055 hasConceptScore W2397360055C2781338088 @default.
- W2397360055 hasConceptScore W2397360055C41183919 @default.
- W2397360055 hasConceptScore W2397360055C45656491 @default.
- W2397360055 hasConceptScore W2397360055C547475151 @default.
- W2397360055 hasConceptScore W2397360055C55493867 @default.
- W2397360055 hasConceptScore W2397360055C71240020 @default.
- W2397360055 hasLocation W23973600551 @default.
- W2397360055 hasLocation W23973600552 @default.
- W2397360055 hasLocation W23973600553 @default.
- W2397360055 hasLocation W23973600554 @default.
- W2397360055 hasOpenAccess W2397360055 @default.
- W2397360055 hasPrimaryLocation W23973600551 @default.
- W2397360055 hasRelatedWork W121172895 @default.
- W2397360055 hasRelatedWork W1260787347 @default.
- W2397360055 hasRelatedWork W1982934610 @default.
- W2397360055 hasRelatedWork W2010996783 @default.
- W2397360055 hasRelatedWork W2046311107 @default.
- W2397360055 hasRelatedWork W2192754539 @default.
- W2397360055 hasRelatedWork W2274217909 @default.
- W2397360055 hasRelatedWork W2312725656 @default.
- W2397360055 hasRelatedWork W2415088446 @default.
- W2397360055 hasRelatedWork W2944114247 @default.
- W2397360055 isParatext "false" @default.
- W2397360055 isRetracted "false" @default.
- W2397360055 magId "2397360055" @default.
- W2397360055 workType "article" @default.