Matches in SemOpenAlex for { <https://semopenalex.org/work/W2397367069> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2397367069 abstract "The purification of prolactin is simplified by the strong binding of the hormone to pituitary tissue. An extreme alkaline or acidic pH is required to effectively extract prolactin and this provides a convenient way of removing a large number of serum and pituitary proteins by an initial extraction near neutrality. The first extraction can be made by homogenization of pituitary glands with O.9% saline (Lewis, Singh, and Seavey, 1971) which is effective for glands of both mammalian and non-mammalian species, or various acidic buffers can be used (Ellis, Grindeland, Nuenke, and Callahan, 1969; Hwang, Robertson, Guyda, and Friesen, 1973). After the initial extraction, prolactin is recovered by treating the tissue with an alkaline buffer (pH 10-11), either aqueous or ethanolic (Ellis et al., 1969; Lewis, Singh, and Seavey, 1972a; Hwang et al., 1973), since prolactin is soluble in this concentration of organic solvent so significant purification can be achieved in a single step. Prolactin can also be extracted from pituitary tissue at acidic conditions (pH 2-3) but this method has not been used frequently. The procedure of Lyons (1937) is the best example of this type of extraction." @default.
- W2397367069 created "2016-06-24" @default.
- W2397367069 creator A5079986874 @default.
- W2397367069 date "1977-01-01" @default.
- W2397367069 modified "2023-10-05" @default.
- W2397367069 title "Chemistry of Prolactin" @default.
- W2397367069 cites W1516520587 @default.
- W2397367069 cites W1584219058 @default.
- W2397367069 cites W1974846095 @default.
- W2397367069 cites W1979287168 @default.
- W2397367069 cites W1982216615 @default.
- W2397367069 cites W1986618242 @default.
- W2397367069 cites W1995444669 @default.
- W2397367069 cites W2003969408 @default.
- W2397367069 cites W2004773891 @default.
- W2397367069 cites W2008528728 @default.
- W2397367069 cites W2011182863 @default.
- W2397367069 cites W2025544237 @default.
- W2397367069 cites W2034135881 @default.
- W2397367069 cites W2042027204 @default.
- W2397367069 cites W2050523516 @default.
- W2397367069 cites W2057164259 @default.
- W2397367069 cites W2057576618 @default.
- W2397367069 cites W2075562111 @default.
- W2397367069 cites W2075758572 @default.
- W2397367069 cites W2159564702 @default.
- W2397367069 cites W2167150519 @default.
- W2397367069 cites W2322960989 @default.
- W2397367069 cites W2468559212 @default.
- W2397367069 doi "https://doi.org/10.1007/978-1-4615-6675-5_2" @default.
- W2397367069 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19946" @default.
- W2397367069 hasPublicationYear "1977" @default.
- W2397367069 type Work @default.
- W2397367069 sameAs 2397367069 @default.
- W2397367069 citedByCount "3" @default.
- W2397367069 crossrefType "book-chapter" @default.
- W2397367069 hasAuthorship W2397367069A5079986874 @default.
- W2397367069 hasConcept C12554922 @default.
- W2397367069 hasConcept C185592680 @default.
- W2397367069 hasConcept C2779064019 @default.
- W2397367069 hasConcept C55493867 @default.
- W2397367069 hasConcept C71315377 @default.
- W2397367069 hasConcept C86803240 @default.
- W2397367069 hasConceptScore W2397367069C12554922 @default.
- W2397367069 hasConceptScore W2397367069C185592680 @default.
- W2397367069 hasConceptScore W2397367069C2779064019 @default.
- W2397367069 hasConceptScore W2397367069C55493867 @default.
- W2397367069 hasConceptScore W2397367069C71315377 @default.
- W2397367069 hasConceptScore W2397367069C86803240 @default.
- W2397367069 hasLocation W23973670691 @default.
- W2397367069 hasLocation W23973670692 @default.
- W2397367069 hasOpenAccess W2397367069 @default.
- W2397367069 hasPrimaryLocation W23973670691 @default.
- W2397367069 hasRelatedWork W1531601525 @default.
- W2397367069 hasRelatedWork W2319480705 @default.
- W2397367069 hasRelatedWork W2384464875 @default.
- W2397367069 hasRelatedWork W2398689458 @default.
- W2397367069 hasRelatedWork W2606230654 @default.
- W2397367069 hasRelatedWork W2607424097 @default.
- W2397367069 hasRelatedWork W2748952813 @default.
- W2397367069 hasRelatedWork W2899084033 @default.
- W2397367069 hasRelatedWork W2948807893 @default.
- W2397367069 hasRelatedWork W2778153218 @default.
- W2397367069 isParatext "false" @default.
- W2397367069 isRetracted "false" @default.
- W2397367069 magId "2397367069" @default.
- W2397367069 workType "book-chapter" @default.