Matches in SemOpenAlex for { <https://semopenalex.org/work/W2397432092> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2397432092 endingPage "S66" @default.
- W2397432092 startingPage "S66" @default.
- W2397432092 abstract "An animal egg such as amphibian, mammalian or sea urchin egg receives only a single sperm at fertilisation. After binding of the first sperm, the egg is prevented from allowing the entry of additional sperm. In fact, polyspermy results in aborted development of the zygote. It has been generally accepted that a molecule(s) released from cortical granules participates in the block to polyspermy. As one such molecule, a cortical granule lectin has been isolated from unfertilised Xenopus eggs ( Xenopus cortical granule lectin; XCGL). XCGL is released into the perivitelline space after fertilisation, and forms a complex with J1 jelly molecules to form an F layer, resulting in a block to additional sperm penetration. A lectin molecule has also been purified from the eggs of several species of fish. The fish egg lectin is located in the cortical alveoli and is released from them after fertilisation. However, its biological function is unclear. We isolated cortical alveolar lectin from unfertilised eggs of Chinook salmon through affinity column chromatography (salmon egg lectin; SEL). The lectin activity was estimated by haemagglutination. The activity of the purified SEL was most strongly inhibited by L-rhamnose and D-galactose, but not by EDTA. Further analysis by C4 reverse-phase column chromatography using HPLC revealed that the lectin was composed of three subunit proteins: 24K, 26Ka and 26Kb proteins. In addition, we cloned cDNAs for them by RT-PCR. The deduced amino acid sequence of the 26Ka protein was homologous with that of the 26Kb protein (identity, 96.4%). Identities of the 24K with the 26Ka and the 26Kb proteins were 55.9% and 66.7%, respectively. A database search revealed that a lectin molecule similar to the SEL had been identified in Anthocidaris crassispina egg (sea urchin egg lectin; SUEL). The SUEL is composed of 105 amino acids, and is similar to both amino-terminal and carboxyl-terminal halves of the SELs. Thus, the SEL molecule is composed of two repeats of such SUEL-like domains, suggesting that the SEL gene was produced by gene duplication." @default.
- W2397432092 created "2016-06-24" @default.
- W2397432092 creator A5031105971 @default.
- W2397432092 creator A5070006545 @default.
- W2397432092 creator A5084034465 @default.
- W2397432092 creator A5084822188 @default.
- W2397432092 creator A5085430631 @default.
- W2397432092 creator A5090880400 @default.
- W2397432092 date "1999-12-01" @default.
- W2397432092 modified "2023-10-14" @default.
- W2397432092 title "Fertilisation in fish: a cortical alveolar lectin and its potential role in the block to polyspermy" @default.
- W2397432092 cites W1969095604 @default.
- W2397432092 cites W2006190875 @default.
- W2397432092 doi "https://doi.org/10.1017/s0967199400130357" @default.
- W2397432092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11191320" @default.
- W2397432092 hasPublicationYear "1999" @default.
- W2397432092 type Work @default.
- W2397432092 sameAs 2397432092 @default.
- W2397432092 citedByCount "5" @default.
- W2397432092 countsByYear W23974320922022 @default.
- W2397432092 countsByYear W23974320922023 @default.
- W2397432092 crossrefType "journal-article" @default.
- W2397432092 hasAuthorship W2397432092A5031105971 @default.
- W2397432092 hasAuthorship W2397432092A5070006545 @default.
- W2397432092 hasAuthorship W2397432092A5084034465 @default.
- W2397432092 hasAuthorship W2397432092A5084822188 @default.
- W2397432092 hasAuthorship W2397432092A5085430631 @default.
- W2397432092 hasAuthorship W2397432092A5090880400 @default.
- W2397432092 hasConcept C104317684 @default.
- W2397432092 hasConcept C153911025 @default.
- W2397432092 hasConcept C159879943 @default.
- W2397432092 hasConcept C179247698 @default.
- W2397432092 hasConcept C181199279 @default.
- W2397432092 hasConcept C185592680 @default.
- W2397432092 hasConcept C196843134 @default.
- W2397432092 hasConcept C19869320 @default.
- W2397432092 hasConcept C202751555 @default.
- W2397432092 hasConcept C2776010925 @default.
- W2397432092 hasConcept C2776690073 @default.
- W2397432092 hasConcept C2779535977 @default.
- W2397432092 hasConcept C2779771984 @default.
- W2397432092 hasConcept C2780758401 @default.
- W2397432092 hasConcept C2781087480 @default.
- W2397432092 hasConcept C2994278967 @default.
- W2397432092 hasConcept C55493867 @default.
- W2397432092 hasConcept C59822182 @default.
- W2397432092 hasConcept C86803240 @default.
- W2397432092 hasConcept C87073359 @default.
- W2397432092 hasConcept C90642696 @default.
- W2397432092 hasConcept C95444343 @default.
- W2397432092 hasConceptScore W2397432092C104317684 @default.
- W2397432092 hasConceptScore W2397432092C153911025 @default.
- W2397432092 hasConceptScore W2397432092C159879943 @default.
- W2397432092 hasConceptScore W2397432092C179247698 @default.
- W2397432092 hasConceptScore W2397432092C181199279 @default.
- W2397432092 hasConceptScore W2397432092C185592680 @default.
- W2397432092 hasConceptScore W2397432092C196843134 @default.
- W2397432092 hasConceptScore W2397432092C19869320 @default.
- W2397432092 hasConceptScore W2397432092C202751555 @default.
- W2397432092 hasConceptScore W2397432092C2776010925 @default.
- W2397432092 hasConceptScore W2397432092C2776690073 @default.
- W2397432092 hasConceptScore W2397432092C2779535977 @default.
- W2397432092 hasConceptScore W2397432092C2779771984 @default.
- W2397432092 hasConceptScore W2397432092C2780758401 @default.
- W2397432092 hasConceptScore W2397432092C2781087480 @default.
- W2397432092 hasConceptScore W2397432092C2994278967 @default.
- W2397432092 hasConceptScore W2397432092C55493867 @default.
- W2397432092 hasConceptScore W2397432092C59822182 @default.
- W2397432092 hasConceptScore W2397432092C86803240 @default.
- W2397432092 hasConceptScore W2397432092C87073359 @default.
- W2397432092 hasConceptScore W2397432092C90642696 @default.
- W2397432092 hasConceptScore W2397432092C95444343 @default.
- W2397432092 hasIssue "S1" @default.
- W2397432092 hasLocation W23974320921 @default.
- W2397432092 hasLocation W23974320922 @default.
- W2397432092 hasOpenAccess W2397432092 @default.
- W2397432092 hasPrimaryLocation W23974320921 @default.
- W2397432092 hasRelatedWork W1511042431 @default.
- W2397432092 hasRelatedWork W1985742112 @default.
- W2397432092 hasRelatedWork W1999583117 @default.
- W2397432092 hasRelatedWork W2009215244 @default.
- W2397432092 hasRelatedWork W2044771514 @default.
- W2397432092 hasRelatedWork W2104584085 @default.
- W2397432092 hasRelatedWork W2152756354 @default.
- W2397432092 hasRelatedWork W237753899 @default.
- W2397432092 hasRelatedWork W2397432092 @default.
- W2397432092 hasRelatedWork W2566759407 @default.
- W2397432092 hasVolume "8" @default.
- W2397432092 isParatext "false" @default.
- W2397432092 isRetracted "false" @default.
- W2397432092 magId "2397432092" @default.
- W2397432092 workType "article" @default.