FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2397579791> ?p ?o ?g. }

• W2397579791 endingPage "1047" @default.
• W2397579791 startingPage "1036" @default.
• W2397579791 abstract "Abstract The epigenetic mechanisms promoting lineage-specific commitment of human skeletal (mesenchymal or stromal) stem cells (hMSCs) into adipocytes or osteoblasts are still not fully understood. Herein, we performed an epigenetic library functional screen and identified several novel compounds, including abexinostat, which promoted adipocytic and osteoblastic differentiation of hMSCs. Using gene expression microarrays, chromatin immunoprecipitation for H3K9Ac combined with high-throughput DNA sequencing (ChIP-seq), and bioinformatics, we identified several key genes involved in regulating stem cell proliferation and differentiation that were targeted by abexinostat. Concordantly, ChIP-quantitative polymerase chain reaction revealed marked increase in H3K9Ac epigenetic mark on the promoter region of AdipoQ, FABP4, PPARγ, KLF15, CEBPA, SP7, and ALPL in abexinostat-treated hMSCs. Pharmacological inhibition of focal adhesion kinase (PF-573228) or insulin-like growth factor-1R/insulin receptor (NVP-AEW51) signaling exhibited significant inhibition of abexinostat-mediated adipocytic differentiation, whereas inhibition of WNT (XAV939) or transforming growth factor-β (SB505124) signaling abrogated abexinostat-mediated osteogenic differentiation of hMSCs. Our findings provide insight into the understanding of the relationship between the epigenetic effect of histone deacetylase inhibitors, transcription factors, and differentiation pathways governing adipocyte and osteoblast differentiation. Manipulating such pathways allows a novel use for epigenetic compounds in hMSC-based therapies and tissue engineering. Significance This unbiased epigenetic library functional screen identified several novel compounds, including abexinostat, that promoted adipocytic and osteoblastic differentiation of human skeletal (mesenchymal or stromal) stem cells (hMSCs). These data provide new insight into the understanding of the relationship between the epigenetic effect of histone deacetylase inhibitors, transcription factors, and differentiation pathways controlling adipocyte and osteoblast differentiation of hMSCs. Manipulating such pathways allows a novel use for epigenetic compounds in hMSC-based therapies for tissue engineering, bone disease, obesity, and metabolic-disorders." @default.
• W2397579791 created "2016-06-24" @default.
• W2397579791 creator A5011905144 @default.
• W2397579791 creator A5014280161 @default.
• W2397579791 creator A5035262769 @default.
• W2397579791 creator A5062897642 @default.
• W2397579791 creator A5076027051 @default.
• W2397579791 creator A5091741741 @default.
• W2397579791 date "2016-05-18" @default.
• W2397579791 modified "2023-09-29" @default.
• W2397579791 title "Epigenetic Library Screen Identifies Abexinostat as Novel Regulator of Adipocytic and Osteoblastic Differentiation of Human Skeletal (Mesenchymal) Stem Cells" @default.
• W2397579791 cites W1502926059 @default.
• W2397579791 cites W1550293405 @default.
• W2397579791 cites W181956002 @default.
• W2397579791 cites W1884290936 @default.
• W2397579791 cites W1970278573 @default.
• W2397579791 cites W1984644927 @default.
• W2397579791 cites W1989857027 @default.
• W2397579791 cites W1991162692 @default.
• W2397579791 cites W1997912521 @default.
• W2397579791 cites W1998226236 @default.
• W2397579791 cites W2013043062 @default.
• W2397579791 cites W2015194229 @default.
• W2397579791 cites W2016701686 @default.
• W2397579791 cites W2020911494 @default.
• W2397579791 cites W2031675589 @default.
• W2397579791 cites W2033205232 @default.
• W2397579791 cites W2035125888 @default.
• W2397579791 cites W2039141069 @default.
• W2397579791 cites W2040404307 @default.
• W2397579791 cites W2041208958 @default.
• W2397579791 cites W2046345187 @default.
• W2397579791 cites W2049601658 @default.
• W2397579791 cites W2052045899 @default.
• W2397579791 cites W2053108708 @default.
• W2397579791 cites W2059361866 @default.
• W2397579791 cites W2064140455 @default.
• W2397579791 cites W2065108395 @default.
• W2397579791 cites W2065625914 @default.
• W2397579791 cites W2072087746 @default.
• W2397579791 cites W2079686283 @default.
• W2397579791 cites W2083124842 @default.
• W2397579791 cites W2088553641 @default.
• W2397579791 cites W2099803844 @default.
• W2397579791 cites W2102313291 @default.
• W2397579791 cites W2102376919 @default.
• W2397579791 cites W2106610488 @default.
• W2397579791 cites W2107264623 @default.
• W2397579791 cites W2107277218 @default.
• W2397579791 cites W2107733280 @default.
• W2397579791 cites W2107793453 @default.
• W2397579791 cites W2108017291 @default.
• W2397579791 cites W2119824866 @default.
• W2397579791 cites W2134865815 @default.
• W2397579791 cites W2136118941 @default.
• W2397579791 cites W2136121675 @default.
• W2397579791 cites W2136499741 @default.
• W2397579791 cites W2143658931 @default.
• W2397579791 cites W2149250742 @default.
• W2397579791 cites W2153720415 @default.
• W2397579791 cites W2159887222 @default.
• W2397579791 cites W2161253076 @default.
• W2397579791 cites W2163341100 @default.
• W2397579791 cites W2192080449 @default.
• W2397579791 cites W2460822283 @default.
• W2397579791 cites W2739174192 @default.
• W2397579791 cites W4211260120 @default.
• W2397579791 doi "https://doi.org/10.5966/sctm.2015-0331" @default.
• W2397579791 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4954455" @default.
• W2397579791 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27194745" @default.
• W2397579791 hasPublicationYear "2016" @default.
• W2397579791 type Work @default.
• W2397579791 sameAs 2397579791 @default.
• W2397579791 citedByCount "24" @default.
• W2397579791 countsByYear W23975797912016 @default.
• W2397579791 countsByYear W23975797912017 @default.
• W2397579791 countsByYear W23975797912018 @default.
• W2397579791 countsByYear W23975797912019 @default.
• W2397579791 countsByYear W23975797912020 @default.
• W2397579791 countsByYear W23975797912021 @default.
• W2397579791 countsByYear W23975797912023 @default.
• W2397579791 crossrefType "journal-article" @default.
• W2397579791 hasAuthorship W2397579791A5011905144 @default.
• W2397579791 hasAuthorship W2397579791A5014280161 @default.
• W2397579791 hasAuthorship W2397579791A5035262769 @default.
• W2397579791 hasAuthorship W2397579791A5062897642 @default.
• W2397579791 hasAuthorship W2397579791A5076027051 @default.
• W2397579791 hasAuthorship W2397579791A5091741741 @default.
• W2397579791 hasBestOaLocation W23975797911 @default.
• W2397579791 hasConcept C101762097 @default.
• W2397579791 hasConcept C104317684 @default.
• W2397579791 hasConcept C134320426 @default.
• W2397579791 hasConcept C137620995 @default.
• W2397579791 hasConcept C148738053 @default.
• W2397579791 hasConcept C150194340 @default.
• W2397579791 hasConcept C167227067 @default.
• W2397579791 hasConcept C190727270 @default.
• W2397579791 hasConcept C198826908 @default.

• next