FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2397631405> ?p ?o ?g. }

• W2397631405 endingPage "C288" @default.
• W2397631405 startingPage "C278" @default.
• W2397631405 abstract "Phosphorylation of the myosin regulatory light chain (LC20-P1) is the major route of smooth muscle activation. However, after prior exposure to vanadate, permeabilized guinea pig taenia coli smooth muscle contracts in the absence of LC20-P1. We characterized the vanadate-induced contraction and investigated the mechanism of this novel activation pathway. Addition of vanadate to a control contracture (6.6 microM Ca2+) inhibits force (effective dose for 50% response was approximately 100 microM). In contrast, preincubation with high concentrations of vanadate (threshold at 1-2 mM) elicited a contraction on subsequent transfer of the fiber to a vanadate-free, Ca(2+)-free solution. Maximum isometric force of approximately 60% of control was obtained in fibers preincubated in 4 mM vanadate for 10 min. Addition of Ca2+ to a vanadate-induced contracture increased force, but the total force never exceeded the initial control. After maximal thiophosphorylation of LC20 with adenosine 5'-O-(3-thiotriphosphate), treatment with vanadate did not increase force. Unloaded shortening velocity (Vmax) was similar in Ca2+ and vanadate contractures and was additive. After thiophosphorylation, preincubation in vanadate had no effect on Vmax, suggesting that vanadate affected the number of activated bridges and not cycle rate. Vanadate mechanisms likely involve oxidation, since preincubation with 4 mM vanadate and 25 mM dithiothreitol (DTT) did not produce force. DTT could reverse a vanadate-induced contracture in 30-60 min. Subsequently, fibers demonstrated control contraction/relaxation cycles. Thus vanadate treatment did not cause irreversible damage, such as the extraction of proteins. Potential oxidation sites are proteins at 17 kDa and between 30 and 40 kDa, which were not alkylated by N-ethylmaleimide if they were treated in the presence of vanadate or in the rigor state. Vanadate-induced contractures are likely mediated by a reversible oxidation that activates cross bridges similarly to that of LC20-Pi and may play an important role in oxidant injury." @default.
• W2397631405 created "2016-06-24" @default.
• W2397631405 creator A5049769324 @default.
• W2397631405 creator A5052113690 @default.
• W2397631405 creator A5058472467 @default.
• W2397631405 date "1997-01-01" @default.
• W2397631405 modified "2023-09-27" @default.
• W2397631405 title "Vanadate oxidation activates contraction in skinned smooth muscle without myosin light chain phosphorylation" @default.
• W2397631405 cites W1505862344 @default.
• W2397631405 cites W1551049760 @default.
• W2397631405 cites W1957276195 @default.
• W2397631405 cites W1979952843 @default.
• W2397631405 cites W1988843900 @default.
• W2397631405 cites W1990405883 @default.
• W2397631405 cites W2002494763 @default.
• W2397631405 cites W2008309107 @default.
• W2397631405 cites W2017480974 @default.
• W2397631405 cites W2017783844 @default.
• W2397631405 cites W2023584868 @default.
• W2397631405 cites W2041506462 @default.
• W2397631405 cites W2045870780 @default.
• W2397631405 cites W2047442562 @default.
• W2397631405 cites W2051432483 @default.
• W2397631405 cites W2054633209 @default.
• W2397631405 cites W2054955273 @default.
• W2397631405 cites W2055978447 @default.
• W2397631405 cites W2058296798 @default.
• W2397631405 cites W2058577093 @default.
• W2397631405 cites W2075012014 @default.
• W2397631405 cites W2076996217 @default.
• W2397631405 cites W2086375139 @default.
• W2397631405 cites W2089742414 @default.
• W2397631405 cites W2092120843 @default.
• W2397631405 cites W2171108012 @default.
• W2397631405 cites W2178203389 @default.
• W2397631405 cites W2224520304 @default.
• W2397631405 cites W2415315408 @default.
• W2397631405 doi "https://doi.org/10.1152/ajpcell.1997.272.1.c278" @default.
• W2397631405 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9038834" @default.
• W2397631405 hasPublicationYear "1997" @default.
• W2397631405 type Work @default.
• W2397631405 sameAs 2397631405 @default.
• W2397631405 citedByCount "3" @default.
• W2397631405 crossrefType "journal-article" @default.
• W2397631405 hasAuthorship W2397631405A5049769324 @default.
• W2397631405 hasAuthorship W2397631405A5052113690 @default.
• W2397631405 hasAuthorship W2397631405A5058472467 @default.
• W2397631405 hasConcept C12554922 @default.
• W2397631405 hasConcept C134018914 @default.
• W2397631405 hasConcept C163415756 @default.
• W2397631405 hasConcept C181199279 @default.
• W2397631405 hasConcept C185592680 @default.
• W2397631405 hasConcept C2776050358 @default.
• W2397631405 hasConcept C2777824588 @default.
• W2397631405 hasConcept C2781116636 @default.
• W2397631405 hasConcept C55493867 @default.
• W2397631405 hasConcept C6997183 @default.
• W2397631405 hasConcept C7301908 @default.
• W2397631405 hasConcept C8035138 @default.
• W2397631405 hasConcept C86803240 @default.
• W2397631405 hasConceptScore W2397631405C12554922 @default.
• W2397631405 hasConceptScore W2397631405C134018914 @default.
• W2397631405 hasConceptScore W2397631405C163415756 @default.
• W2397631405 hasConceptScore W2397631405C181199279 @default.
• W2397631405 hasConceptScore W2397631405C185592680 @default.
• W2397631405 hasConceptScore W2397631405C2776050358 @default.
• W2397631405 hasConceptScore W2397631405C2777824588 @default.
• W2397631405 hasConceptScore W2397631405C2781116636 @default.
• W2397631405 hasConceptScore W2397631405C55493867 @default.
• W2397631405 hasConceptScore W2397631405C6997183 @default.
• W2397631405 hasConceptScore W2397631405C7301908 @default.
• W2397631405 hasConceptScore W2397631405C8035138 @default.
• W2397631405 hasConceptScore W2397631405C86803240 @default.
• W2397631405 hasIssue "1" @default.
• W2397631405 hasLocation W23976314051 @default.
• W2397631405 hasLocation W23976314052 @default.
• W2397631405 hasOpenAccess W2397631405 @default.
• W2397631405 hasPrimaryLocation W23976314051 @default.
• W2397631405 hasRelatedWork W1996012468 @default.
• W2397631405 hasRelatedWork W2024549821 @default.
• W2397631405 hasRelatedWork W2039328609 @default.
• W2397631405 hasRelatedWork W2042878884 @default.
• W2397631405 hasRelatedWork W2088927121 @default.
• W2397631405 hasRelatedWork W2140994672 @default.
• W2397631405 hasRelatedWork W2159852460 @default.
• W2397631405 hasRelatedWork W2397631405 @default.
• W2397631405 hasRelatedWork W2400526927 @default.
• W2397631405 hasRelatedWork W3104526097 @default.
• W2397631405 hasVolume "272" @default.
• W2397631405 isParatext "false" @default.
• W2397631405 isRetracted "false" @default.
• W2397631405 magId "2397631405" @default.
• W2397631405 workType "article" @default.