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- W2397716449 abstract "For many years, cell therapies have been hampered by limited availability and inter-batch variability of primary cells. Human embryonic stem cell (hESC) can give rise to specialized cells like keratinocytes and recently emerged as a virtually unlimited source of potential therapeutic cells. However, xenogeneic components in differentiation cocktails have been limiting the clinical potential of hESC-derived keratinocytes (hESCs-Kert). Here, we demonstrated efficient differentiation of H9 human embryonic stem cells (H9-hESCs) into keratinocytes (H9-KertACC) in an autogenic co-culture system. We used activin as the main factor to induce keratinocyte differentiation. H9-KertACC expressed keratinocyte markers at mRNA and protein levels. Establishment of such animal-free microenvironment for keratinocyte differentiation will accelerate potential clinical application of hESCs." @default.
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- W2397716449 date "2013-01-01" @default.
- W2397716449 modified "2023-10-18" @default.
- W2397716449 title "Differentiation of Epidermal Keratinocytes from Human Embryonic Stem Cells" @default.
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- W2397716449 doi "https://doi.org/10.1007/7651_2013_46" @default.
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