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- W2398047078 abstract "[FeFe]-hydrogenases are nature's most prolific hydrogen catalysts, excelling at facilely interconverting H2 and protons. The catalytic core common to all [FeFe]-hydrogenases is a complex metallocofactor, referred to as the H-cluster, which is composed of a standard [4Fe-4S] cluster linked through a bridging thiolate to a 2Fe subcluster harboring dithiomethylamine, carbon monoxide, and cyanide ligands. This 2Fe subcluster is synthesized and inserted into [FeFe]-hydrogenase by three maturase enzymes denoted HydE, HydF, and HydG. HydE and HydG are radical S-adenosylmethionine enzymes and synthesize the nonprotein ligands of the H-cluster. HydF is a GTPase that functions as a scaffold or carrier for 2Fe subcluster production. Herein, we utilize UV-visible, circular dichroism, and electron paramagnetic resonance spectroscopic studies to establish the existence of redox active [4Fe-4S] and [2Fe-2S] clusters bound to HydF. We have used spectroelectrochemical titrations to assign iron-sulfur cluster midpoint potentials, have shown that HydF purifies with a reduced [2Fe-2S] cluster in the absence of exogenous reducing agents, and have tracked iron-sulfur cluster spectroscopic changes with quaternary structural perturbations. Our results provide an important foundation for understanding the maturation process by defining the iron-sulfur cluster content of HydF prior to its interaction with HydE and HydG. We speculate that the [2Fe-2S] cluster of HydF either acts as a placeholder for HydG-derived Fe(CO)2CN species or serves as a scaffold for 2Fe subcluster assembly." @default.
- W2398047078 created "2016-06-24" @default.
- W2398047078 creator A5028675962 @default.
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- W2398047078 date "2016-06-14" @default.
- W2398047078 modified "2023-09-24" @default.
- W2398047078 title "A Redox Active [2Fe-2S] Cluster on the Hydrogenase Maturase HydF" @default.
- W2398047078 cites W1538196126 @default.
- W2398047078 cites W1941641374 @default.
- W2398047078 cites W1963921746 @default.
- W2398047078 cites W1966488638 @default.
- W2398047078 cites W1967418107 @default.
- W2398047078 cites W1971396352 @default.
- W2398047078 cites W1973208024 @default.
- W2398047078 cites W1981403174 @default.
- W2398047078 cites W1983783030 @default.
- W2398047078 cites W1984339790 @default.
- W2398047078 cites W1985889211 @default.
- W2398047078 cites W1986394490 @default.
- W2398047078 cites W1987395294 @default.
- W2398047078 cites W1989252386 @default.
- W2398047078 cites W1996711495 @default.
- W2398047078 cites W1998953863 @default.
- W2398047078 cites W2000494671 @default.
- W2398047078 cites W2004583631 @default.
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- W2398047078 cites W2008711518 @default.
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- W2398047078 cites W2027622313 @default.
- W2398047078 cites W2027861057 @default.
- W2398047078 cites W2040760712 @default.
- W2398047078 cites W2041616745 @default.
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- W2398047078 doi "https://doi.org/10.1021/acs.biochem.6b00528" @default.
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- W2398047078 hasPublicationYear "2016" @default.
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